乙肝免疫球蛋白不能阻断乙肝病毒感染人绒毛膜癌细胞

目的: 观察不同浓度乙肝免疫球蛋白(HBIG)在体外对乙肝病毒(HBV)感染人绒毛膜癌细胞系JAR细胞的影响。方法: 实验分为HBIG实验组、空白对照组(胎牛血清)、阴性对照组(健康人血清)。HBIG实验组又分为高浓度HBIG组(HBV+10³IU/L HBIG、HBV+10² IU/L HBIG)、最低保护浓度HBIG组(HBV+10 IU/L HBIG)、低浓度HBIG组(HBV+1IU/L、HBV+0.1 IU/L)和HBV组;采用MTT法检测HBIG对细胞生长的影响;采用实时荧光定量PCR检测细胞内HBV-DNA的表达;采用细胞免疫荧光染色法检测细胞内乙肝表面抗原(HBsAg)的表达变化。结果: (1) 不同浓度的HBIG对JAR细胞生长无影响;(2) 不同浓度HBIG组的细胞内HBsAg的荧光强度无显著差异(P>0.05);(3)不同浓度HBIG组的细胞内HBV-DNA无显著差异(P> 0.05)。结论: HBIG不能阻断HBV感染体外培养的JAR细胞。

Hepatitis B immungolobulin has no blocking effect on hepatitis B virus infection of choriocarcinoma cell line JAR in vitro

AIM: To study the blocking effects of hepatitis B immunoglobulin(HBIG) on hepatitis B virus(HBV) infection of choriocarcinoma JAR cells in vitro.METHODS: The JAR cells were divided into experimental groups,blank control group(using fetal bovine serum) and negative control group(using healthy human serum).The experimental groups were divided into high concentration groups of HBIG(HBV +103 IU/L HBIG,or HBV +102 IU/L HBIG),minimum protective concentration group of HBIG(HBV+10 IU/L HBIG),low concentration groups of HBIG(HBV+1 IU/L HBIG,or HBV+0.1 IU/L HBIG) and HBV group.The effect of HBIG on the cell growth was analyzed by MTT assay.HBV-DNA in JAR cells was determined by real-time fluorescence quantitative PCR.The expression of hepatitis B surface antigen(HBsAg) in JAR cells was detected by immunofluorescent staining.RESULTS: Different concentrations of HBIG had no effect on the growth of JAR cells.No different of HBV-DNA copies in JAR cells infected with HBV and treated with different concentrations of HBIG was observed(P>0.05).No difference of fluorescent intensity of HBsAg in JAR cells with HBV infection and treated with different concentrations of HBIG was found(P>0.05).CONCLUSION: HBIG dose not block the HBV infection of JAR cells in vitro.