同源结构域相互作用蛋白激酶2在宫颈癌中的表达及其与人乳头瘤病毒感染和细胞凋亡的关系

目的 探讨宫颈癌中同源结构域相互作用蛋白激酶2(HIPK2)的表达及其与人乳头瘤病毒(HPV)感染和细胞凋亡的关系.方法 取50例宫颈癌组织和15例正常宫颈组织,采用免疫组化法测定HIPK2和HPV的表达,采用DNA原位末端标记(TUNEL)技术检测了细胞凋亡情况.结果 15例正常宫颈组织中,HIPK2、HPV和PCNA阳性表达者分别有1、3和4例,而50例宫颈癌组织中分别有44、39和45例,官颈癌组织HIPK2、HPV和PCNA的表达明显高于正常官颈组织(均P<0.05).宫颈癌组凋亡指数(AJ)为(1.6±0.7)%,正常宫颈组AI为(5.7±0.2)%,差异有统计学意义(P<0.01).在宫颈癌组织中,HIPK2表达与HPV表达呈正相关(r=0.467,P=0.0006),与AI呈负相关(r=-0.370,P=0.0083).结论 在宫颈癌中,HIPK2的表达与HPV感染和细胞凋亡密切相关,可能在宫颈癌发生、发展中起重要作用.

Correlation of HIPK2 expression with HPV infection and apoptosis in cervical cancer

Objective To evaluate the correlation of the expression of homeodomain-interacting protein kinase 2 (HIPK2) with human papillomavirus (HPV) infection and apoptosis in cervical cancer. Methods Formalin-fixed, paraffin embedded tissue samples from 50 cervical cancers and 15 normal uterine cervix cases were obtained. Apoptosis was quantified by TdT-mediated dUTP nick end labeling (TUNEL) assay and the expression of HIPK2 as well as HPV by immunohistochemical staining. Results HIPK2 protein expression was detected in 88.0% (44/50) of cervical cancers and 6.7% ( 1/15 ) of normal cervical tissues. HPV was found in 78.0% (39/50) of cervical cancers and 20. 0% (3/15) of normal cervical tissue samples. The expression of HIPK2 protein was significantly and positively correlated with HPV presence (r = 0. 467, P < 0. 01 ), but negatively with apoptotic index ( r = - 0. 370, P < 0. 05 ). Conclusion HIPK2 protein expression is positively correlated with HPV infection, but negatively with apoptotic index in cervical cancers. Therefore, HIPK2 may be involved in the mechanism of apoptosis in cervical cancer and may play an important role in cervical carcinogenesis.