安多霖对高功率微波辐射大鼠生精细胞凋亡的影响及机制研究

目的 探讨安多霖对微波辐射的防护作用及作用机制。方法 选用SPF级雄性SD大鼠,随机分为3个给药组,剂量分别为3、6、9 g/kg,另设辐射模型组和健康对照组,每组20~21只。给药组大鼠连续灌胃安多霖20 d ,停药后,给药组和辐射模型组动物行一次平均功率密度为100 mW/cm²高功率微波全身辐照10min,健康对照组不辐照。动物分别于辐照后24、48 h和5 d 取睾丸组织,制作睾丸石蜡切片,采用原位末端标记术(TUNEL)检测睾丸生精细胞凋亡,免疫组化法检测细胞凋亡相关蛋白Bax和Bcl-2。结果 微波辐射后24、48 h和5 d,与健康对照组相比,辐射组睾丸生精细胞的凋亡数明显升高(t=-41.89、-11.29、-62.24,P<0.05),Bcl-2/Bax比值明显降低(t=8.49、4.36、4.47,P<0.05);而与辐射组相比,低、中和高剂量给药组的凋亡细胞数显著降低(F=5.25、9.79、15.35, P<0.05), Bcl-2/Bax比值明显升高(F=20.17、11.75、11.98, P<0.05)。结论 高功率微波辐射可诱导大鼠生精细胞凋亡增加,安多霖对细胞凋亡有明显抑制作用。安多霖抑制大鼠睾丸生精细胞凋亡的机制可能与其能够上调Bcl-2及下调Bax蛋白的表达,改变了Bcl-2/Bax的比值有关。

Effect of Auduolin on apoptosis and expression of Bax and Bcl-2 in spermatogenic cells of rats exposed to high power microwave

Objective To investigate the protection function of Anduolin (ADL) against exposure to high power microwave, and to probe the mechanism involved. Methods The SPF-class male rats were randomly divided into ADL groups (Dose of 3, 6 and 9 g/kg body weight), model group and control group. Rats in ADL groups were continuously ingested with ADL for 20 d. The rats in ADL groups and model group were exposed to high power microwave at average power density of 100 mW/cm² for 10min. Their testes were excised at 24 h, 48 h and 5 d after exposure to microwave. Apoptosis of spermatogenic cells and the expression of Bax and Bcl-2 were detected with in situ end-labeling method and immuno-histochemistry method, respectively. Results At 24 h,48 h and 5 d after exposure to high power microwave, compared with control group, the counts of apoptotic spermatogenic cells in exposed model group were increased significantly(t=-41.89,-11.29 and -62.24,P<0.05), while the ratio of Bcl-2/Bax in exposed group was decreased significantly(t=8.49,4.36 and 4.47, P<0.05). At the same time, compared with the exposed group, the counts of apoptotic spermatogenic cells in 3, 6, and 9 g/kg ADL groups were reduced significantly(F=5.25,9.79 and 15.35, P<0.05), while the ratio of Bcl-2/Bax was enhanced significantly(F=20.17,11.75 and 11.98, P<0.05). Conclusions The high power microwave could induce the apoptosis of spermatogenic cells. ADL protect the spermatogenic cells via the ratio Bcl-2/Bax increasing.