丹参多酚酸对糖尿病大鼠血液流变学的改善作用及其机制研究

目的考察丹参多酚酸对糖尿病大鼠血液流变学的改善作用,并探讨其作用机制。方法 SD大鼠随机分为对照组和模型组,其中对照组10只,模型组30只。进行糖尿病模型诱导后,造模成功的大鼠随机分为模型组以及丹参多酚酸1.79、5.26 mg/kg组,每组10只。丹参多酚酸组ip注射用丹参多酚酸盐1.79、5.26 mg/kg(以丹参乙酸镁计),模型组、对照组ip同体积蒸馏水,给药容积均为2.76 m L/kg,1次/d,连续给药14 d。取血测定血液流变学指标和红细胞膜表面生物学特性指标。结果与模型组比较,丹参多酚酸1.79、5.26 mg/kg组全血黏度3、全血黏度50、红细胞电泳时间、血沉方程K值均显著降低(P0.05、0.01)。与模型组比较,丹参多酚酸1.79、5.26 mg/kg组唾液酸含量显著升高(P0.05),丹参多酚酸5.26 mg/kg组Na+-K+-ATP酶活性、SOD水平均显著升高(P0.05)。结论丹参多酚酸盐通过增加红细胞膜表面唾液酸含量、提高红细胞膜抗氧化能力来改善糖尿病大鼠的血液流变学特性。

Improvement of salvianolic acids on hemorheology of diabetic rats and its mechanism

Objective To study the improvement of salvianolic acids on hemorheology of diabetic rats and to explore its mechanism.Methods SD rats were randomly divided into control and model groups. And there were 10 rats in the control group, and 30 rats in the model group. Diabetes model were induced. Diabetic rats were randomly divided into model group and salvianolic acids (1.79 and 5.26 mg) groups, and each group had 10 rats. Rats in salvianolic acids groups were ip administered with Depsides Salts from Salvia miltiorrhiza in Lyophilizied Powder for injection 1.79 and 5.26 mg (magnesium acetate as index), and those in control and model groups were ip administered with the same volume of normal saline. The volume was 2.76 mL/kg, the treatment were carried out once daily, and lasted for 14 d. The indexes of hemorheology and surface characteristics of erythrocyte membrane were determined.Results Compared with the model group, the whole blood viscosity 3, whole blood viscosity 50, erythrocyte electrophoretic time, and ESR equation K values in salvianolic acids (1.79 and 5.26 mg/kg) groups were significantly decreased (P < 0.05, 0.01). Compared with the model group, the contents of sialic acid in salvianolic acids (1.79 and 5.26 mg/kg) groups were significantly increased (P < 0.05), and Na⁺-K⁺-ATP enzyme activities and SOD levels in salvianolic acids (5.26 mg/kg) group were significantly increased (P < 0.05).Conclusion Salvianolic acids can increase sialic acid contents of red cell surface, enhance antioxidant capacity of erythrocyte membrane, and improve hemorheology characteristics of diabetic rats.