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| MK基因特异性siRNA对乳腺癌细胞凋亡的影响 | |
| 引用本文： | 孙梅,刘洪义,张兴义,李玉林. MK基因特异性siRNA对乳腺癌细胞凋亡的影响[J]. 中国实验诊断学, 2009, 13(4): 427-429 |
| 作者姓名： | 孙梅 刘洪义 张兴义 李玉林 |
| 作者单位： | 1. 吉林大学白求恩医学部,病理生物学教育部重点实验室,吉林,长春,130021 2. 吉林大学第一临床医院,普通外科,吉林,长春130021 3. 吉林大学第二临床医院,胸外科,吉林,长春,130041 |
| 摘 要： | 目的探讨RNA干扰技术抑制Midkine对人乳腺癌癌细胞株MCF-7细胞凋亡的影响。方法人工合成抑制Midkine基因的siRNA片段，通过脂质体转染到MCF-7细胞内，应用RT-PCR检测bcl-2mRNA表达，流式细胞术检测细胞线粒体膜电位变化，通过测定光密度值检测Caspase-3活性。结果转染siRNA后的MCF-7细胞，bcl-2mRNA表达显著下降、细胞线粒体膜电位明显下降和Caspase-3酶活性升高。结论MK-siRNA通过下调hcl-2基因，而改变细胞线粒体膜电位使Caspase-3酶活性升高诱导细胞的凋亡。 |
| 关 键 词： | siRNA Midkine MCF-7 凋亡 |
Effects of Midkine siRNA on apoptosis of MCF-7 cell |
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| Affiliation: | SUNMei,LIUHong-yi,ZHANGXing-yi,etal.(1.MEKeyLaboratory of Pathobiology , School of Bethune Medical Sciences, Jilin University, Changchun 130021, China ; 2. Department of General Surgery, First Hospital of Jilin University, Changchun 130021, China ;3. Department of Thoracic Surgery,, Second Hospital of Jilin University, Chongchun 130041, China) |
| Abstract: | Objective To explore the apoptosis of MCF-7 cells induced by siRNA-mediated midkine silencing. Methods The selected siRNA targeting Midkine （MK） was transfected into MCF-7 cells by LipofectaminTM2000. The expression of bcl-2 mRNA was detected by RT-PCR.The mitochondrial membrane potential was determined by flow cytmeter analysis and the activity of caspase-3 was examined by colorirnctric. Results For the MCF-7 cell transfected with siRNA, the expression of Bcl-2 mRNA was significantly decreased. The mitechondrial membrane potential was loss and the activity of caspase-3 was increased. Contusion siRNA targeting MK gene can induce apoptosis via down-regulation of Bcl-2, mitochondrial depolarization and caspase-3 activation. |
| Keywords: | siRNA Midkine MCF-7 |
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