高效液相色谱法测定豨莶通栓丸中三七皂苷R1、人参皂苷Rg1和Rb1的含量

目的：建立HPLC法测定豨莶通栓丸中三七皂苷R1、人参皂苷Rg1及Rb1含量的方法。方法采用C18色谱柱；流动相：乙腈（A）-水（B）梯度洗脱；流速：1.0 ml/min，检测波长为203 nm。结果三七皂苷R1、人参皂苷Rg1及Rb1的线性范围分别为0.412～1.855μg（r=0.9994）、1.903～8.563μg（r=0.9994）、1.822～8.201μg（r=1.0000）；总体平均回收率分别为98.48%、97.33%和97.29%，其RSD分别为1.58%、1.13%和1.08%。结论本法可靠、准确，可用于该制剂的质量控制。

Determination of notoginsenoside R1,ginsenosides Rg1 and Rb1 in Xixian Tongshuan pills by HPLC

Objective To establish a HPLC method for determining notoginsenoside R1,and ginsenosides Rg1 and Rb1 in Xixian Tongshuan pills. Methods C18 column was adopted with acetonitrile as mobile phase A,water as mobile phase B,gradient elution. The flow rate was 1.0 ml/min,column temperature was 35℃and detection wavelength was 203 nm. Results The linear range of notoginsenoside R1,and ginsenosides Rg1 and Rb1 was 0.412-1.855μg(r=0.9994),1.903-8.563μg(r=0.9994),and 1.822-8.201μg(r=1.0000),respectively. The total average recovery rate of notoginsenoside R1,and ginsenosides Rg1 and Rb1 was 98.48%, 97.33%and 97.29%with RSD of 1.58%,1.13%and 1.08%,respectively. Conclusion The method is reliable,accurate,and can be used for the quality control of the preparation.