丙泊酚对电离辐射引起小鼠造血系统损伤的防护作用

目的：观察丙泊酚对电离辐射引起造血系统损伤防护作用。方法实验采用3种照射剂量：生存率实验照射剂量为7.5 Gy、脾结节形成实验照射剂量为6 Gy、外周血细胞计数和骨髓单侧股骨细胞计数实验照射剂量为2 Gy；生存率实验分为4组：7.5Gy组、7.5Gy+5 mg/kg丙泊酚组、7.5Gy+10 mg/kg丙泊酚组、7.5Gy+20 mg/kg丙泊酚组。其余实验分为4组：对照组、丙泊酚20 mg/kg组、照射组（2Gy或6Gy）、照射+丙泊酚20 mg/kg组。给药方式为照射前1 d、照射当天提前30 min各给药一次和照射后7d连续腹腔注射，每日1次，照射后第9天活杀小鼠，检测脾结节形成数，各类外周血细胞数及单侧股骨骨髓细胞数。结果丙泊酚20 mg/kg能提高受照射小鼠30 d生存率，增加受照射小鼠脾结节形成数、增加受照射小鼠外周血白细胞数、红细胞数、血红蛋白含量及小鼠单侧股骨骨髓有核细胞数，与照射对照组比较，结果有统计学意义（P＜0.05）。结论丙泊酚对电离辐射引起的造血系统损伤均有保护作用，其作用机制有待进一步研究。

Protective effect of propofol on total body irradiation induced hematopoietic system injury in mice

Objective To observe the protective effect of propofol on hematopoietic system injury in mice with total body irra?diation(TBI). Method Three different radiation doses were used in the experiments:7.5 Gy TBI in 30 day-survival experiment,6Gy TBI in colony-forming unit spleen(CFU-S)experiment and 2Gy TBI in the other experiment;mice were divided into 4 groups in a 30 day-survival experiment,including 7.5Gy TBI group,7.5 Gy TBI+5 mg/kg propofol group,7.5 Gy TBI+10 mg/kg propofol group and 7.5 Gy TBI + 20 mg/kg propofol group. For the other experiments,mice were divided into 4 groups:control group,propofol group,TBI(2 or 6 Gy)group,and TBI+20 mg/kg propofol group. Propofol of 20 mg/kg were administered to mice 1 d before TBI,30 mins before TBI and once each day within the following 7 days after TBI. Mice were euthanized on the ninth day after TBI,the number of CFU-S,peripheral blood parameters and bone marrow cells per femur were measured in this experiment. Results Propofol im?proved the 30 day-survival of lethally irradiated mice. There were increases in number of CFU-S,white blood cells,red blood cells, hemoglobin in peripheral blood and bone marrow cells per femur in 2 Gy TBI+20 mg/kg propofol group compared to 2 Gy TBI group (P<0.05). Conclusion Propofol exhibits a promising protective effect on TBI-induced hematopoietic system injury;further study should be focused on the related mechanisms.