麻疹、腮腺炎、风疹和水痘联合减毒活疫苗中水痘病毒滴度测定方法研究

目的 建立并验证麻疹、腮腺炎、风疹和水痘(measles,mumps,rubella and varicella,MMRV)联合减毒活疫苗中水痘病毒滴度的测定方法.方法 首先通过比较温度,确定中和条件;再根据抗麻疹、腮腺炎、风疹病毒血清对相应病毒的完全中和能力,确定每种抗血清的使用浓度.观察抗血清对2BS细胞生长的影响和对水痘病毒的干扰作用.采用t检验对结果进行比较.结果 与37℃1h相比,4℃1h的中和条件能准确反映MMRV疫苗中水痘病毒的滴度水平(t=6.7082,P＜0.01).3种抗血清(麻疹1∶80、腮腺炎1∶40、风疹1∶40)混合后对2BS细胞生长无影响,对不同滴度水痘病毒无干扰(高滴度t=0.4472,P＞0.05;中滴度t=0.9045,P＞0.05;低滴度t=0.3536,P＞0.05).使用建立的方法测定MMRV疫苗中水痘病毒滴度,实测值与理论值之间的差异无统计学意义(t=1.7533,P ＞0.05).结论 建立了MMRV联合减毒活疫苗中水痘病毒滴度的测定方法.

Study on a titration method of varicella virus in live attenuated measles,mumps, rubella and varicella combined vaccine

Objective To establish and verify a titration method for varicella virus in live attenuated measles,mumps,rubella and varicella (MMRV) combined vaccine.Methods The neutralization condition was determined by temperature comparison and the appropriate concentration of anti-sera against measles,mumps,and rubella viruses was adopted according to the neutralization ability of each anti-serum to the corresponding virus.The influence on 2BS cell growth and interference with varicella virus by anti-sera were observed.The results were compared by t-test.Results Compared with 37℃ 1 h,the neutralization condition of 4℃ 1 h reflected accurately the titer of varicella virus in MMRV vaccine (t=6.7082,P ＜0.01).Three kinds of anti-sera (measles 1∶80,mumps 1∶40,rubella 1∶40) had no influence on 2BS cells.No interference of anti-sera with different titer varicella viruses was observed (high titer t=0.4472,P ＞0.05;middle titer t=0.9045,P ＞0.05;low titer t=0.3536,P ＞0.05).There was no significant difference between the measured and theoretical titers of varicella virus (t=1.7533,P ＞0.05).Conclusion The method for determining varicella virus in MMRV vaccine is established.