白及提取物对博莱霉素致大鼠肺纤维化的治疗作用

目的：探究白及提取物中抗大鼠肺纤维化（PE）的活性部位，并对活性部位的化学性质进行分析。方法采用95%乙醇提取白及得到白及醇提物，药渣水提醇沉法提取得到白及多糖。雄性SD大鼠气管内一次性注射博莱霉素（5 mg/kg）诱导PF模型，灌胃给予白及醇提物（300 mg/kg）或白及多糖（300 mg/kg），14 d和28 d观察肺组织病理学及胶原沉积变化，计算肺系数，并检测羟脯氨酸含量。采用苯酚-硫酸法测定白及多糖部分糖含量，高效凝胶色谱法测定其相对分子质量大小，高效液相色谱方法研究其单糖组成。结果与PE模型组比较，白及多糖治疗组的大鼠肺组织病理改变明显改善，且肺系数及羟脯氨酸含量明显降低（P＜0.05，P＜0.01），而白及醇提物组无显著性改善作用。白及多糖糖含量81.41%，数均分子质量（Mn）约为2.23×105，由甘露糖和葡萄糖组成。结论白及多糖能显著降低肺组织羟脯氨酸的产生及胶原的沉积，并延缓PE的发展进程。

Inhibitory effect of Bletilla striata extracts on bleomycin-induced pulmonary fibrosis in rats

Objective To investigate the inhibitory effect of Bletilla striata extracts(BSE)on bleomycin-induced pulmonary fibrosis(PF)in rats and study the chemical characteristics of the active component. Methods B. striata was extracted with 95%ethyl?alcohol to obtain the ethanol extract. The residue was extracted with water and precipitated with ethanol to obtain the B. striata polysac?charide(BSP). Sprague Dawley male rats were intratracheally administered of bleomycin(BLM,5 mg/kg)to induce PF. PF Rats were administered with ethanol BSE(300 mg/kg body mass)and BSP(300 mg/kg body mass)once daily for 28 d by gavage. On 14 d and 28 d, the lungs were harvested to observe the pathological and collagen deposition changes. The lung coefficient and the content of hydroxy?proline(HYP)were also determined. The phenol-sulfuric acid method was used to detect the total sugar content,the relative molecular mass was detected by high performance gel permeation chromatography(HPGPC)and the monosaccharide components were analyzed by high performance liquid chromatography(HPLC). Results Compared with PF model group,the histopathological changes in lung tissue treated with BSP group were obviously improved ,the level of HYP in lung tissues and the lung coefficient were significantly decreased (P<0.05,P<0.01). But the group treated with ethanol BSE had no significant improvement. The content of BSP was 81.41%,and the number average molecular weight(Mn)of BSP was about 2.23 × 105. The BSP was mainly composed of glucose and mannose. Conclusion HYP could significantly decrease lung HYB and collagen deposition of PF rats,and has the potiential for inhi?bition of PF.