| 低氧诱导心肌细胞表达血管内皮生长因子发生机制的实验研究 |
| |
| 引用本文： | 吴钢,郝亚荣,李庚山,黄从新,王晶,谢强,黄峥嵘. 低氧诱导心肌细胞表达血管内皮生长因子发生机制的实验研究[J]. 微循环学杂志, 2004, 14(1): 8-9,12 |
| |
| 作者姓名： | 吴钢 郝亚荣 李庚山 黄从新 王晶 谢强 黄峥嵘 |
| |
| 作者单位： | 武汉大学人民医院心内科,武汉,430060 |
| |
| 基金项目： | 湖北省九五科技攻关项目 ( 962 9110 1) |
| |
| 摘 要： | 目的 :研究低氧诱导心肌细胞表达分泌血管内皮生长因子(VEGF)的发生机制。方法 :将新生SD大鼠的心肌细胞进行原代培养 ,培养的细胞分为二组 :正常对照组和低氧培养组 ,低氧组采用向培养瓶内灌注 99.99%N2 的方法 ,2 4h后将预置的盖玻片取出 ,进行免疫组织化学检测分裂原活化蛋白激酶 (MAPK)活性。结果 :低氧培养的心肌细胞MAPK活性高于对照组 (P <0 .0 0 1)。结论 :MAPK通路可能是低氧促进心肌细胞表达和分泌VEGF蛋白的机制之一
|
| 关 键 词： | 心肌细胞 血管内皮生长因子 机制 大鼠 低氧诱导 |
| 文章编号： | 1005-1740(2004)01-0008-03 |
Experimental Studies on the Mechanism of Development for the Expression of Cardiomyocytes on VEGF by Induced Hypoxia in Rat |
| |
| Wu Gang,Hao Yarong,Li Gengshan,et al/. Experimental Studies on the Mechanism of Development for the Expression of Cardiomyocytes on VEGF by Induced Hypoxia in Rat[J]. Chinese Journal of Microcirculation, 2004, 14(1): 8-9,12 |
| |
| Authors: | Wu Gang Hao Yarong Li Gengshan et al/ |
| |
| Affiliation: | Wu Gang,Hao Yarong,Li Gengshan,et al/Department of Cardiology,Renmin Hospital of Wuhan University,Wuhan 430060 |
| |
| Abstract: | Objective: To study experimental studies on the mechanism of development for the expression of cardiomyocytes on VEGF by induced hypoxia in rat.Method: The neonatal rat cardiomyocytes were primary cultured. These cardiomyocytes were divided into 2 groups: normal culture group and hypoxia-induced group. The hypoxic group was done by irrigating 99.99% N 2. After 24 hours, the cover glasses being lay up in advance were gained. The MAPK expression was measured by immunohistochemistry.Results: MAPK expression increased significantly in cultured cardiomyocytes exposed hypoxia.Conclusion: MAPK pathway is one of the mechanism of VEGF expression and secretion in cardiomyocytes. |
| |
| Keywords: | Cardiomyocytes Vascular endothelial growth factor(VEGF) Mechanism Rat Hypoxia induced |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
