贲门癌、胃癌患者红细胞C3b受体与CD4+/CD8+比值、NK细胞活性的相关性研究

背景与目的:研究贲门癌、胃癌患者红细胞C3b受体(RBC-C3bR)与CD4 /CD8 比值、NK细胞活性的相关性。材料与方法:利用酵母菌花环试验、克隆抗体致敏花环法和MTT法对53例贲门癌、51例胃癌患者及30例正常人外周血RBC-C3bR阳性率、T细胞CD3 、CD4 百分率、CD4 /CD8 比值及自然杀伤细胞(NK)活性进行测定。结果:贲门癌组、胃癌组RBC-C3bR阳性率、CD3 、CD4 细胞百分率、CD4 /CD8 比值和NK细胞杀伤活性均比正常对照组低,差异具有统计学意义(F=28.68,P<0.01),而贲门癌与胃癌组间比较,差异无统计学意义(t=0.081～0.871,P均>0.05);经统计学相关性分析显示,贲门癌、胃癌组RBC-C3bR阳性率与CD4 /CD8 比值、NK细胞杀伤活性间呈正相关(贲门癌RBC-C3bR阳性率与CD4 /CD8 比值:r=0.431,P<0.01;RBC-C3bR阳性率与NK细胞活性:r=0.347,P<0.05;胃癌RBC-C3bR阳性率与CD4 /CD8 比值:r=0.429,P<0.01;RBC-C3bR阳性率与NK细胞活性:r=0.528,P<0.01)。结论:贲门癌、胃癌患者红细胞免疫与白细胞免疫功能均受到抑制。

Red Blood C3b Receptor and Ratio f CD4+/CD8+and NK Cell Activity in Gastric Cardia Carcinoma and Gastric Cancer Patients

BACKGROUND AND AIM:To investigate the correlation between red blood C3b receptor(RBC-C3bR) and lymphocytic cell(TC) immune function in tumor patients.MATERIALS AND METHODS:RBC-C3bR,natural killer(NK) cell activity and T lymphocyte subsets of 53 patients with gastric cardia carcinoma,51 gastric cancer and 30 healthy controls were measured by microzyme garland test,MTT-assay and monoclonal anti-human T lymphocyte antibody.RESULTS:Compared with the control group,RBC-C3bR,NK cell activity,CD3 ,CD4 ,CD4 /CD8 ratio were significantly decreased in tumor patients(F=28.68,P<0.01).Positive correlations were observed between RBC-C3bR with both NK cell activity and CD4 /CD8 ratio(gastric cardia carcinoma RBC-C3bR and CD4 /CD8 ratio:r=0.431,P<0.01;RBC-C3bR and NK cell activity:r=0.347,P<0.05;gastric cancer RBC-C3bR and CD4 /CD8 ratio:r=0.429,P<0.01;RBC-C3bR and NK cell activity,r=0.528,P<0.01).CONCLUSION:The immune funtion of erythrocytes was inhibited same as lymphocytes and there was close relationship between them in gastric cardia carcinoma,gastric cancer patients.