应用中脑细胞微团培养技术探讨双酚A的发育毒性

目的 为揭示双酚A(BPA)是否具有神经系统发育毒性及其毒作用机制提供实验依据。方法 采用微团培养技术观察不同浓度的BPA(0—60mg／L)对体外培养的13d龄Wistar大鼠胚胎中脑细胞分化的影响及其细胞毒性作用。结果 BPA对体外培养的大鼠胚胎中脑细胞的存活和分化均有抑制作用并呈现出明显的剂量反应关系。BPA对中脑细胞的50％细胞存活抑制浓度(ICV50)和50％分化抑制浓度(ICD50)分别为57．2mg/L和24．6mg／L。两者比值为2.33。结论 BPA对大岚胚胎中脑细胞分化的抑制作用大于对细胞存活的抑制作用。按照Flint等人推荐的体外致畸分类标准，BPA属于阳性致畸物。

The developmental toxicity of bisphenol A in micromass culture of rat embryonic midbrain cell

Objective To evaluate the characteristic of developmental toxicity and its mechanism of bisphenol A (BPA) in vitro . Methods Thirteen day Wistar rat embryo midbrain (central nervous system, CNS) were exposed to BPA at concentrations of 0,10,20,30,40,50, or 60 mg/L in the culture medium. Results When midbrain cells were treated with BPA for 5 days, induction of cytotoxi city and inhibition of cell differentiation were observed in a concentration dependent manner. The higher level of BPA appeared to be cytotoxic to Wistar rat embryo midbrain cells in culture and reduced the number of differentiated foci with dose relationship. The IC 50 value of BPA for cytotoxicity and cell differentiation were 57 2 mg/L and 24 6 mg/L respectively. Conclusion The results indicated that BPA induced developmental toxicity in rat embryonic midbrain cells and the data suggested that the specific induction of cytotoxicity and inhibition of cell differentiation might be one of the mechanisms of high level BPA in vitro . According to Flints's teratogenic criteria, BPA might be a potential teratogen.