定量PCR检测黄芪A6组分与无环鸟苷联合抗1型单纯疱疹病毒的协同作用

目的：探讨抗疱疹病毒药物黄芪Ａ６组分（Ａ６）和无环鸟苷（ＡＣＶ）联合抗１型单纯疱疹病毒（ＨＳＶ１）的作用机制。方法：利用竞争ＰＣＲ检测Ａ６和ＡＣＶ联合抗ＨＳＶ１的协同作用，并与细胞病变（ＣＰＥ０抑制法进行比较。结果：竞争ＰＣＲ测定Ａ６、ＡＣＶ和Ａ６＿ＡＣＶ对ＨＳＶ１的最小抑制浓度（ＭＩＣ）分别为１．８８ｍｇ／ｍｌ、３．３７μｇ／ｍｌ、３．３７μｇ／ｍｌ和０．４７ｍｇ／ｍｌ＋０．８４μｇ／ｍｌ；ＣＰ

Quantitative study on synergistic effect of radix astragali A6 and acyclovir against herpes simplex virus type I by polymerase chain reaction]

OBJECTIVE: To evaluate the synergistic effect of Radix Astragali A6(A6) and acyclovir (ACV) in antagonizing herpes simplex virus type I (HSV1). METHODS: The synergistic effect was measured by competitive polymerase chain reaction (PCR) and compared with that measured by traditional cytopathic effect (CPE) inhibition method. RESULTS: The minimum inhibition concentration of A6, ACV and A6 + ACV measured by PCR was 1.88 mg/ml, 3.37 micrograms/ml and 0.47 mg/ml + 0.84 microgram/ml respectively, while measured by CPE, it was 6.25 mg/ml, 50 micrograms/ml and 0.94 mg/ml + 12.5 micrograms/ml respectively. The fractional inhibitory combined indexes were all less than 0.5, which is an indication of obvious co-synergistic effect. CONCLUSION: The quantitative PCR is an effective method of screening antiviral drugs. The inhibitory action of A6 and ACV on HSV1 revealed mainly at the replicative stage of viral proliferation cycle.