雷洛昔芬对去卵巢大鼠骨密度及血E2、IL-6水平影响的研究

目的观察雷洛昔芬对去势大鼠骨密度、血清E2、IL-6及子宫内膜的影响，探讨雷洛昔芬抗骨质疏松的作用机制。方法48只3个月龄雌性SD大鼠，随机分为SHAM组（假手术），0VX组（单纯去势），0VX＋E2组（去势＋雌激素），0VX＋R组（去势＋雷洛昔芬），每组12只。3个月后处死采集血，用放射免疫分析法检测并比较各组E2及IL-6的表达，取子宫内膜用HE染色法观察各组子宫内膜的形态变化并对子宫内膜腺体数目进行统计学分析。结果雷洛昔芬组大鼠骨密度显著高于去卵巢组（P＜0．05），血清IL-6浓度值显著降低，雌激素浓度的改变差异无统计学意义（与去卵巢组比较P＞0．05），对子宫内膜无明显影响（P＞0．05）。结论雷洛昔芬治疗骨质疏松的作用与血清中IL-6水平降低相关，与雌激素无显著相关，对子宫内膜无显著影响。

Effect of raloxifene on the bone density and sera estradiol and interleukin-6 in ovariectomized rats

Objective To explore the effect of raloxifene on bone density, sera E2 and IL-6, uterus endometrium in ovariectomized rats. Methods Forty-eight female Sprague-Dawley rats, aged 3 months were evenly divided into 4 groups randomly: sham group underwent sham ovariectomy, OVX group underwent bilateral ovariectomies, OVX E2 group underwent bilateral ovariectomies followed by estrogens treatment, OVX R group underwent ovariectomies followed by raloxifene treatment. These rats were killed after 3 months. The sera E2 and IL-6 were measured by radioimmunoassay. The endometrium was observed under microscope after HE staining. The bone density was tested by radioimn counter. Results The serum IL-6 in OVX R Group was significantly higher than OVX group (P<0.05), but serum E2 endometrium remained no different. Conclusion Raloxifene may play role in treatment of osteoporosis via decrease serum IL-6, but unrelated to E2 and not resulting in proliferation of uterus endometrium.