Urinary Nucleic Acid TSPAN13-to-S100A9 Ratio as a Diagnostic Marker in Prostate Cancer |
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Authors: | Chunri Yan Ye-Hwan Kim Ho Won Kang Sung Phil Seo Pildu Jeong Il-Seok Lee Dongho Kim Jung Min Kim Yung Hyun Choi Sung-Kwon Moon Seok Joong Yun Wun-Jae Kim |
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Affiliation: | 1.Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea.;2.Bio-Medical Science Co. Ltd, Daejeon, Korea.;3.Nucleic Acid Research Center, Inc., Daejeon Oriental Hospital of Daejeon University, Daejeon, Korea.;4.Department of Biochemistry, Dong-Eui University, Busan, Korea.;5.Department of Food and Biotechnology, Chung-Ang University, Seoul, Korea. |
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Abstract: | The potential use of urinary nucleic acids as diagnostic markers in prostate cancer (PCa) was evaluated. Ninety-five urine samples and 234 prostate tissue samples from patients with PCa and benign prostatic hyperplasia (BPH) were analyzed. Micro-array analysis was used to identify candidate genes, which were verified by the two-gene expression ratio and validated in tissue mRNA and urinary nucleic acid cohorts. Real-time quantitative polymerase chain reaction (qPCR) was used to measure urinary nucleic acid levels and tissue mRNA expression. The TSPAN13-to-S100A9 ratio was selected to determine the diagnostic value of urinary nucleic acids in PCa (P = 0.037) and shown to be significantly higher in PCa than in BPH in the mRNA and nucleic acid cohort analyses (P < 0.001 and P = 0.013, respectively). Receiver operating characteristic (ROC) analysis showed that the area under the ROC curve was 0.898 and 0.676 in tissue mRNA cohort and urinary nucleic acid cohort, respectively. The TSPAN13-to-S100A9 ratio showed a strong potential as a diagnostic marker for PCa. The present results suggest that the analysis of urine supernatant can be used as a simple diagnostic method for PCa that can be adapted to the clinical setting in the future. |
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Keywords: | Prostatic Neoplasms Cell-free Nucleic Acid Urine Two-gene Expression Ratio S100A9 TSPAN13 Diagnostic Marker |
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