The correlation of results of panel reactive antibody,identification, and single antigen beads in detection of anti-HLA antibodies: Istanbul Faculty of Medicine,tissue typing laboratory experience |
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| Institution: | 1. Istanbul University, Istanbul Faculty of Medicine, Department of Medical Biology, Tissue Typing Laboratory, Istanbul-Turkiye, Millet street Fatih, Istanbul 34093, Turkey;2. Istanbul University, Institute of Health Sciences/Medical Biology, Istanbul, Turkiye;1. Clinical Research Support Center, Mie University Hospital, 2-174 Edobashi, Tsu, Mie 514-8507, Japan;2. Department of Pharmacy, Mie University Hospital, 2-174 Edobashi, Tsu, Mie 514-8507, Japan;3. Organ Transplantation Centre, Mie University Hospital, 2-174 Edobashi, Tsu, Mie 514-8507, Japan;1. Section of Dermatology, Department of Health Sciences, University of Genoa, Policlinico San Martino, Via R. Benzi, 10, 16132 Genoa, Italy;2. Section of Dermatology, Department of medical and surgical sciences, University of Foggia, Policlinico Riuniti, Viale Pinto 1, 71122 Foggia, Italy;3. Physical medicine and rehabilitation, Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics and Maternal Child Health (DINOGMI), University of Genoa, Genoa, Largo P. Daneo 3, 16132 Genoa, Italy;4. Liver Unit, C.U.R.E., Department of Medical and Surgical Sciences, University of Foggia, Viale Pinto 1, 71122 Foggia, Italy;5. Section of Dermatology, Department of Dermatology and Angioma Center, IRCCS Istituto Giannina Gaslini, Via G. Gaslini, 5, 16147 Genoa, Italy;1. School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran;2. Functional Neurosurgery Research Center, Shohada Tajrish Neurosurgical Center of Excellence, Shahid Beheshti University of Medical Sciences, Tehran, Iran;1. The Aga Khan University - Medical College, The Aga Khan University, Karachi, Pakistan;2. Baylor College of Medicine, Houston, TX, United States of America;3. New York University School of Medicine, Department of Internal Medicine, United States of America;4. Division of Nephrology and Abdominal Transplantation, Department of Medicine, Baylor College of Medicine, Houston, TX, United States of America;5. Division of Abdominal Transplantation, Department of Surgery, Baylor College of Medicine, Houston, TX, United States of America.;1. Department of Urology, The Second Hospital of Lanzhou University, Lanzhou, China;2. Gansu Province Clinical Research Center for Urology, Lanzhou, China;3. Second Clinical School, Lanzhou University, Lanzhou, China |
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| Abstract: | BackgroundWe have performed a retrospective analysis of anti-HLA class I MHC and class II MHC antibodies measured using a single antigen bead (SAB) assay and a panel reactive antibody (PRA) assay.Material and methodsA group of 256 patients with end-stage renal disease (ESRD) was tested for anti-HLA antibodies in the tissue typing laboratory between 2017 and 2020. In the cohort, the serum samples of patients waiting for transplantation were tested. Both the PRA and SAB tests of these patients were analyzed using the Luminex (Immucor) method. The threshold of positivity was accepted as median fluorescence intensities (MFI) ≥1000 for PRA screening and MFI ≥750 for SAB screening.ResultsOverall, antibodies to HLA antigens were detected in 202 (78.9%) out of 256 patients in the PRA study. Antibodies against both class I/II antigens were detected only in 15.6% of these patients, whereas antibodies against only against class I HLA in 31.3% and only against class II HLA in 32.0%. By comparison, the SAB study found that 66.8% of patients were positive for HLA antigens. Furthermore, donor-specific antibodies (DSA) were detected in 52.0% of PRA-positive patients and 52.6% of SAB-positive patients. It was shown that 168 patients (83.2%) out of 202 PRA-positive patients were found to be SAB-positive. In addition, 51 patients negative in the SAB assay (94.4%) were also negative in the PRA assay. Statistical analysis established a significant correlation between the PRA and SAB positivity (p > 0.001).It was also shown that MFI ≥3000 PRA positivity for class I HLA antigens (p = 0.049) and MFI ≥5000 PRA positivity for class II antigens (p < 0.001) correlated with the SAB positivity in patients.ConclusionOur results showed the importance of both PRA and SAB assays to define the status of sensitization in patients. |
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