PINK1 and oxidative stress in lean and obese patients with type 2 diabetes mellitus |
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| Affiliation: | 1. MD (Biochemistry), Senior Resident, Department of Biochemistry, University College of Medical Sciences & GTB Hospital, University of Delhi, Delhi, India;2. MD (Biochemistry), Professor, Department of Biochemistry, University College of Medical Sciences & GTB Hospital, University of Delhi, Delhi, India;3. DM (Endocrinology), Professor, Department of Endocrinology, University College of Medical Sciences & GTB Hospital, University of Delhi, Delhi, India;4. MD (Biochemistry), Professor, Department of Biochemistry, University College of Medical Sciences & GTB Hospital, University of Delhi, Delhi, India;5. MD (Biochemistry), Director Professor and Head, Department of Biochemistry, University College of Medical Sciences & GTB Hospital, University of Delhi, Delhi, India;1. Department of Basic Sciences, College of Medicine and Health Sciences, Mohammed Bin Rashid University (MBRU), Dubai, United Arab Emirates;2. Internal Medicine Unit, “Vittorio Emanuele II” Hospital, Castelvetrano, Trapani, Italy;3. Department of Biomedicine, Neuroscience, and Advanced Diagnostics, Institute of Clinical Biochemistry, Clinical Molecular Medicine, and Laboratory Medicine, University of Palermo, Palermo, Italy;4. Department of Laboratory Medicine, University Hospital "P. Giaccone", Palermo, Italy;5. Department of Diabetes, Nutrition, and Metabolic Diseases, Carol Davila University of Medicine, Bucharest, Romania;6. “Prof. Dr.N.C.Paulescu” National Institute of Diabetes, Nutrition and Metabolic Diseases, Bucharest, Romania;7. Department of Medicine, University of Central Florida College of Medicine, Orlando, FL, USA;8. School of Medicine, Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties (Promise), University of Palermo, Italy;1. Department of Echocardiography of The Third Affiliated Hospital of Soochow University, Chang Zhou City, Jiangsu Province, China;2. Department of Endocrinology of The Third Affiliated Hospital of Soochow University, Chang Zhou City, Jiangsu Province, China;1. Department of Research Operations and Diabetes Complications, Madras Diabetes Research Foundation, Chennai, India;2. NIHR Biomedical Research Centre, Moorfields Eye Hospital NHS Foundation Trust, London, UK;3. Vision Sciences, UCL Institute of Ophthalmology, London, UK;4. Department of Bio-Statistics, Madras Diabetes Research Foundation, Chennai, India;5. Department of Diabetology, Madras Diabetes Research Foundation & Dr. Mohan''s Diabetes Specialities Centre, Chennai, India;6. Giridhar Eye Institute, Cochin, Kerala, India;7. Department of Ophthalmology, Madras Diabetes Research Foundation & Dr. Mohan''s Diabetes Specialities Centre, Chennai, India;1. Greg Brown Diabetes and Endocrine Research Laboratory, Sydney Medical School (Central), Faculty of Medicine and Health, Charles Perkin Centre, The University of Sydney, Sydney, NSW, Australia;2. Department of Endocrinology, Royal Prince Alfred Hospital, Sydney, NSW, Australia;3. Nepean Clinical School, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia |
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| Abstract: | AimTo compare mRNA [messenger RNA] expression of PINK1 in whole blood and the levels of biomarkers of Oxidative Stress (mitochondrial DNA [mtDNA] content & Total Antioxidant status [TAS]) in newly diagnosed lean and obese patients with T2DM.MethodsNewly diagnosed patients of T2DM were enrolled in this study. The patients were divided into two groups of 30 patients each, lean (BMI < 18.5 kg/m2) and obese (BMI > 25 kg/m2). mRNA expression of PINK1 & mtDNA content was measured by real time PCR. Serum TAS was measured using a commercially available kit.ResultsThere was a 1.78-fold decrease in mRNA expression of PINK1 in obese group compared to the lean group. Mean mtDNA content was 300.82 ± 169.66 in the obese group and 332.78 ± 147.07 in the lean group (p = 0.06). Mean levels of TAS was 5.39 ± 2.28 μM Trolox Equivalents in the obese group and 3.85 ± 3.33 μM Trolox Equivalents in the lean group (p = 0.001).ConclusionThe T2DM patient with obesity had greater OS than the lean patients. Thus, there is a compensatory increase in antioxidants in obese patients with T2DM. Our findings also suggest that decreased levels of PINK1 in obese group are unable to protect the mitochondria against OS leading to decreased mtDNA content. Does it also result in beta cell dysfunction or contribute to insulin resistance in obese patients with T2DM needs to be explored. |
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