| 高糖环境对培养血管内皮细胞NO、ET-1、MDA和SOD水平的影响 |
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| 引用本文： | 肖静,黄俊. 高糖环境对培养血管内皮细胞NO、ET-1、MDA和SOD水平的影响[J]. 江西医学院学报, 2008, 48(1): 26-28,32 |
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| 作者姓名： | 肖静 黄俊 |
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| 作者单位： | [1]南昌大学研究生院医学部2005级,南昌330006 [2]南昌大学第一附属医院心内科,南昌330006 |
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| 摘 要： | 目的通过对不同浓度葡萄糖刺激下人脐静脉内皮细胞条件培养上清中一氧化氮（NO）活力、内皮素（ET）含量、丙二醛（MDA）含量及超氧化物歧化酶（SOD）活力的检测，探讨高糖对内皮细胞的影响及与血管收缩因子内皮素、舒张因子一氧化氮、血氧自由基和自由基的清除剂超氧化物歧化酶之间的关系。方法采用硝酸还原酶法测定NO含量，硫代巴比妥酸比色法测定MDA含量，放射免疫学方法测定ET含量，黄嘌呤氧化酶法测定SOD含量。结果在高糖处理的细胞条件培养上清中SOD、NO活性降低，而MDA及ET含量升高（P〈0．05）。结论高糖可诱导内皮细胞活性氧产生增多，抗氧化酶活性下降，促进内皮细胞释放ET，抑制NO合成。
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| 关 键 词： | 高糖 人脐静脉内皮细胞 一氧化氮 内皮素 丙二醛 超氧化物岐化酶 |
| 文章编号： | 1000-2294（2008）01-0026-03 |
| 收稿时间： | 2007-07-27 |
| 修稿时间： | 2007-07-27 |
Effect of High Glucose Environment on Cell Viability,NO,ET-1,MDA and SOD in Cultured Endothelial Cells |
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| XIAO Jinga,HUANG Junb. Effect of High Glucose Environment on Cell Viability,NO,ET-1,MDA and SOD in Cultured Endothelial Cells[J]. Acta Academiae Medicinae Jiangxi, 2008, 48(1): 26-28,32 |
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| Authors: | XIAO Jinga HUANG Junb |
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| Affiliation: | XIAO Jinga,HUANG Junb(a.Medical Department 2005,Gvaduate Academy,b.Department of Cardiology,First Affiliated Hospital,Nanchang University,Nanchang 330006,China) |
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| Abstract: | Objective To detect the nitric oxide(NO),endothelin(ET),malondialdehyde(MDA)and superoxide dismutase(SOD)in cultured endothelial cells exposure to different glucose concentration,and investigate the effect of high glucose concentration ET,SOD,NO and MDA.Methods The levels of NO,MDA,ET,and SOD in the supernatants were detected by nitrate reductase test,thiobarbituric acid test,radioimmunity method and xanthine oxidase test,respectively.Results When endothelial cells were cultured in high glucose,the activiti... |
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| Keywords: | high glucose human umbilical vein endothelial cell nitric oxide malondialdehyde endothelin super oxide dismutase |
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