| 根尖周炎动物模型中CD14、TLR4的表达 |
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| 引用本文： | 张琛,侯本祥.根尖周炎动物模型中CD14、TLR4的表达[J].中华口腔医学杂志,2007,42(3):148-149. |
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| 作者姓名： | 张琛 侯本祥 |
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| 作者单位： | 首都医科大学口腔医学院牙体牙髓科,100050 |
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| 基金项目： | 北京市科技新星计划（H013610290113） |
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| 摘 要： | 目的研究大鼠根尖周炎炎症组织中脂多糖炎症信号受体CD14、Toll样受体4（Toll．1ikereceptor4，TLR4）的表达特点，探讨根尖周炎中脂多糖的信号转导途径。方法建立大鼠磨牙内毒素根尖周炎模型，采用免疫组化染色观察根尖周炎炎症组织中CD14、TLR4的表达情况，并计算CD14、TLR4的阳性细胞率。结果正常根尖周组织中未发现CD14和TLR4免疫阳性细胞，根尖周炎症组织中CD14和TLR4表达阳性，CD14、TLR4阳性细胞率差异无统计学意义（P〉0．05）。结论与正常根尖周组织相比，炎症根尖周组织中CD14和TLR4的表达显著增强，CD14和TLR4的表达量差异无统计学意义，提示脂多糖可能通过CD14、TLR4信号受体在根尖周炎症中发挥作用。
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| 关 键 词： | 根尖周疾病 脂多糖类 抗原 CD14 |
| 收稿时间： | 2006-11-22 |
Expression of lipopolysacharide receptors CD14 and TLR4 in a model of rat molar apical periodontitis |
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| ZHANG Chen,HOU Ben-xiang.Expression of lipopolysacharide receptors CD14 and TLR4 in a model of rat molar apical periodontitis[J].Chinese Journal of Stomatology,2007,42(3):148-149. |
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| Authors: | ZHANG Chen HOU Ben-xiang |
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| Institution: | Department of Cariology and Endodontology, Faculty of Stomatology, Capital University of Medical Sciences, Beijing 100050, China. |
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| Abstract: | OBJECTIVE: To characterize the expression of lipopolysaccharide (LPS) receptors-CD14 and TLR4 in rat periradicular tissue and to investigate the signal transduction pathway of LPS and its receptors in periapical periodontitis. METHODS: After establishment of a model of rat molar LPS-induced apical periodontitis, CD14 and TLR4 expression in healthy and inflammatory periapical tissue were determined by immunohistochemistry. RESULTS: CD14 and TLR4 were not detected in healthy periapical tissue but strongly positive in inflammatory periapical tissue. Positive cells were mostly monocytes/macrophages. CONCLUSIONS: CD14 and TLR4 may play an important role in the pathogenesis of periapical periodontitis, probably via immune cells such as monocytes/macrophages and neutrophils. |
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| Keywords: | Periapical diseases Lipopolysacharides Antigens CD14 |
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