视网膜移植抗原性的初步研究

目的：探讨视网膜的免疫原性，为视网膜移植的免疫排斥提供理论依据。方法：采用C57BL/6小鼠，提供视网膜全层、感光细胞层，受体为BALB/C小鼠，并设立阳性及阴性对照组，测定脚掌皮肤迟发性超敏反应及细胞毒性淋巴细胞功能。
结果：①异种全视网膜移植引起了迟发性超敏反应(P<0.05)，而异种感光细胞移植未引起迟发性超敏反应(P>0.05)。②异种全视网膜移植后产生了一定的细胞毒性淋巴细胞功能，在1：90浓度下的杀死率为33.4%，明显高于阴性对照组(同种脾细胞为5.3%，同种全视网膜为4.8%，P<0.05)，而经过抗CD₈单克隆抗体处理后其毒性淋巴细胞功能消失。 结论：异种全层视网膜有一定的移植免疫原性，而异种感光细胞移植可能没有明显的移植免疫原性。 （中华眼底病杂志，1997，13：234-236）

PRELIMINARY STUDY ON IMMUNOGENICITY OF THE RETINA

PURPOSE:To carry out preliminary study on immunogenicity or''the retina and provide theoretical bassis of transplant rejection of the retina. METHODS:The allogeneic whole retinal or photoreceptor layer from C57BL/6 mice wer transplanted subcutaneously into BALB/C mice for antigen exposure and delayed hypersensitivity (DH) and modified ⁵¹Cr-release assay for specific cytotoxic T lymphoeytes (CTL)were emploied. RESULTS:The allogeneic whole retinal transplantation gave rise to DH(P<0.05 )and increased function of CTL of which the killing rate was 33.4% in concentration of 1:90 comparing with negative group (4.8% in 1:90,P<0.05)and the risen function could be blocked by anti-CD_8. CONCLUSION:We deduce that allogeneic whole retina has immunogenicity and should pay attention to transplant rejection postoperatively.but the photoreceptor layer seems to have no immunogenicity and may be no transplant rejection after its transplantation. （Chin J Ocul Fundus Dis,1997,13: 234-236）