鼻粘膜呼吸区上皮细胞原代培养模型的建立和纤毛运动频率的测量

目的：探讨建立人鼻粘膜呼吸区上皮细胞原代培养模型和纤毛运动频率测量的方法。方法：利用酶消化法分离、以无血清生长因子培养基培养人鼻粘膜上皮细胞。用电视显微镜法观察并记录纤毛运动。结果：培养的占膜呼吸上皮细胞在接种后２４ｈ贴壁，６￣８ｄ汇合，成活１６ｄ，因实验需要结束培养。纤毛细胞纤毛摆动活跃，杯状细胞既分泌酸性粘多吼分泌中性粘多糖，所培养细胞２３对４６条染色体正常。用电视显微镜法测得２９例１４５个人

Culture of human nasal respiratory epithelial cells in serum free medium supplemented with hormones and growth factors and measurement of human nasal ciliary motility using videomicroscopy

OBJECTIVE: To establish a culture model of human nasal respiratory epithelial cells and a method of measuring human nasal ciliary motility. METHOD: The human nasal respiratory epithelial cells were detached with collagenase and cultured in serum free medium supplemented with hormones and growth factors, the ciliary beat frequency was measured by videomicroscopy. RESULT: After inoculation, cells cultured with this method adhered in 24 hours, confluented in 6-8 days and lived for 16 days. During that time ciliary beating was active, both acidic and neutral mucoitin granules were rich in goblet cells and all chromosome of 23 pairs were normal, the ciliary beat frequency in 29 subjects' nasal mucosa was (411 +/- 24) beats/min (mean +/- s). CONCLUSION: A culture model of human nasal respiratory epithelial cells in serum free medium supplemented with hormones and growth factors and a method of measuring human nasal ciliary motility was successfully established.