福建厦门微小牛蜱中埃立克体的检测与鉴定

目的 检测福建厦门微小牛蜱携带的埃立克体，进行分类鉴定。方法 采用套式PCR方法检测埃立克体，然后对阳性标本中的埃立克体进行16SrRNA全基因序列测定并分析。结果 共检测吸血微小牛蜱标本299份，27份阳性，阳性率为9．0％。选择其中的1份阳性样本扩增16SrRNA的全基因序列，全长1458bp，序列对比显示与泰缅边境和越南的埃立克体同源性为99．9％，相差2个碱基；与西藏埃立克体同源性为99．7％，相差4个碱基；与非洲埃立克体同源性为99．6％，相差7个碱基；与中国南方的查菲埃立克体同源性为98．9％，相差17个碱基。结论 从福建厦门微小牛蜱中检测到埃立克体，可能是一个埃立克体新的亚种。

Detection and identification of Ehrlichia sp.in Boophilus microplus ticks from Xiamen of Fujian Province

Objective To detect and identify the Ehrlichia pathogens from Boophilus microplus ticks collected from Xiamen in Fujian Province. Methods A nested PCR specific for tick-transmitted Ehrlichia sp. was established with 3 pair of primers designed based on the 16S rRNA sequence of Ehrlichia. The 16S rRNA sequence of Ehrlichia in a representative fragments sample was determined and analyzed. Results 27(9.0%) of 299 B. microplus were detected positive. A representative sample was selected for 16S rRNA gene sequence. A sequenced 1 458 base pairs was obtained. The sequence similarity of the newly detected Fujian Ehrlichia sp. was 99.9% to that of Ehrlichia sp. EBm52 from the Thai-Myanmar border and Vietnam, 99.8% to that of Ehrlichia sp. from Tibet of China, 99.6% to Ehrlichia sp. ERm58 from Africa, 98.9% to the E. chaffensis from Southern China. Conclusion This is the first report that Ehrlichia sp. was detected in B. microplus from Fujian. The agent detected in the study is probably a new subspecies.