莫诺苷对缺血再灌注大鼠皮层TIMP表达的影响

目的研究莫诺苷对缺血再灌注7 d大鼠皮层中金属基质蛋白酶组织抑制剂(tissue inhibitor of metalloproteinase,TIMP)表达的影响。方法 25只雄性SD大鼠采用改良Zealonga线栓法制备大鼠大脑中动脉阻塞(middle cerebral artery occlusion,MCAO)模型,术后随机分为模型组、莫诺苷小剂量组、中剂量组、大剂量组,每组5只。造模3 h后按30、90、270 mg/kg剂量每天一次灌胃莫诺苷。免疫印迹法(Western Blotting)和免疫荧光染色法(Immunofluorencent staining)分析莫诺苷对脑缺血再灌注7 d大鼠患侧皮层TIMP表达的影响。结果脑缺血再灌注7 d后,与假手术组相比,模型组TIMP蛋白表达显著升高(P0.01);同模型组相比,莫诺苷中剂量组(90 mg/kg)和大剂量组(270 mg/kg)TIMP蛋白表达均明显升高(P0.05;P0.01)。结论莫诺苷可以上调TIMP在大脑皮层内的表达,有助于脑卒中后血脑屏障的保护及修复。

Effects of morroniside on the expression of the TIMP in peri-infarct cortex after cerebral ischemia-reperfusion in rats

Objective To study the effects of morroniside on the expression of the tissue inhibitor of metalloproteinase (TIMP) of the ischemic ipsilateral cortex 7 days after ischemia reperfusion. Methods 25 male Sprague-Dawley rats were subjected to middle cerebral artery occlusion (MCAO) model with modified Zea Longa's method, and then divided them into sham group (n=5), ischmia group (n=5), and morroniside groups (low, medium, and high dosage groups, n=5) randomly. Morroniside were then administered intragastrically once a day at dose of 30 mg/kg, 90 mg/kg and 270 mg/kg 3 hours after operation. The expression of TIMP of the ischemic ipsilateral cortex on 7 days after ischemia-reperfusion were detected by western blotting and immunofluorescent staining analysis. Results Compared with the sham group, the expression of TIMP in ischemia group increased (P < 0.05) 7 days after MCAO. Compared with the ischemia group, after treatment with morroniside at doses of 90 mg/kg and 270 mg/kg, the expression of TIMP increased significantly (P < 0.05; P < 0.01). Conclusions Morroniside could increase the expression of TIMP in the ischemic ipsilateral cortex and may contribute to blood-brain barrier protection and restoration.