刀豆蛋白A诱导小鼠肝损伤模型的血清蛋白质差异分析

目的 研究刀豆蛋白(Con)A诱导小鼠肝损伤模型血清中蛋白质的变化,分析特异蛋白质与疾病进展的关系.方法 25只小鼠分为空白对照组,PBS对照组,ConA诱导肝损伤模型1、3、6 h组,取血清,运用ProteoExtract~(TM)白蛋白去除试剂盒纯化小鼠血清,去除白蛋白,利用双向凝胶电泳和质谱技术分析鉴定差异性表达蛋白质.结果 通过对各组双向凝胶电泳图像的比较分析发现,ConA诱导肝损伤模型6 h组有2种明显差异表达的蛋白质.经质谱鉴定,这2种蛋白质分别为血清淀粉样蛋白酶A2前体蛋白和血清淀粉样蛋白酶A1前体蛋白.结论 在ConA诱导小鼠肝损伤模型6 h时,发现血清淀粉样蛋白酶A2前体蛋白和血清淀粉样蛋白酶A1前体蛋白,可能与疾病进展有关.

Analysis of differential expression of serum proteins in concanavalin A induced liver injury mouse model

Objective To study the differential expression of serum proteins in concanavalin A(ConA) induced liver injury mouse model and analyze the relationship between disease progression and special proteins. Methods Twenty-five male mice were randomly divided into five groups, i.e. blank control group, phosphate-buffered saline (PBS) control group, liver injury groups developed 1, 3 and 6 h after ConA injection. The sera from all five groups were removed off the albumin by ProteoExtract~(TM)albumin removal kit. Then two dimensional electrophoresis ( 2DE) and mass spectrometry analysis were utilized to identify differences in protein expressions. Results Two specific proteins were detected in the liver injury group developed 6 h after ConA injection, which were identified as serum amyloid A-2 protein precursor and serum amyloid A-l protein precursor by mass spectrometry. Conclusions Serum amyloid A-2 protein precursor and serum amyloid A-l protein precursor are found at 6 h of ConA injection in ConA induced liver injury mouse model, which may be related to disease progression.