节肢动物生物安全二级实验室蚊虫感染相关的生物安全风险及控制

医学媒介节肢动物（arthropods of medical importance）如蚊、蜱、白蛉等是虫媒传染病流行暴发的关键驱动因素之一,给全球公共卫生安全带来巨大威胁。为了进一步了解虫媒传染病的传播机制,以便更好地制定防控措施,需在实验室条件下开展媒介节肢动物与病原体相互作用研究。为了确保实验研究过程中的生物安全,除实验室硬件设施提供的物理防护外,还需要全面识别实验操作过程中的生物安全风险,并制定相应的风险控制措施,以消除或降低由于操作不当所造成的生物安全风险。本文以蚊虫感染生物危害2类（risk group 2）病原体为例,介绍了在节肢动物生物安全二级（arthropod containment level-2, ACL-2）实验室内操作医学媒介蚊虫和病原体的实践经验,为我国相关领域的科研人员提供参考。

Biosafety risks and mitigation strategies for mosquito infection in Arthropod Containment Level-2 laboratory

Arthropods of medical importance such as mosquitoes, ticks and sandflies are one of the key drivers of arthropod-borne diseases outbreak, posing a great threat to global public health security. For further understanding the transmission mechanisms of arthropod-borne diseases and establishing the prevention and control measures, a series of experiments of arthropods infection need to be carried out under laboratory conditions. Besides the regular biosafety requirements, some specific considerations need to be taken into account when performing arthropod infection and the infected arthropod rearing. Except for the physical containment composed of biosafety facilities, a comprehensive assessment of the biosafety risks during operations and corresponding preventive measures are also critical to eliminate or mitigate the biosafety risks. In this paper, we introduce our practice in handling mosquito infection with Risk Group 2 pathogens in Arthropod Containment Level-2 (ACL-2) laboratory, with an aim to provide a reference for researchers in related fields. © 2023 China Tropical Medicine. All rights reserved.