鼻息肉中嗜酸性粒细胞浸润和活化状况的研究

目的 :观察鼻息肉组织中嗜酸性粒细胞浸润和活化状况 ,探讨嗜酸性粒细胞在鼻息肉发病机制中的作用。方法 :16例鼻息肉组织标本 ,采用组织化学染色 Chromotrope 2R染色法 ,标记鼻息肉组织中的嗜酸性粒细胞 ,结合应用嗜酸性粒细胞阳离子蛋白抗体EG2 的免疫组织化学染色结果 ,观察鼻息肉中嗜酸性粒细胞的浸润和活化状况。结果 :①鼻息肉组织中有较多嗜酸性粒细胞浸润 ,且多处于活化状态 ;②变应性患者鼻息肉组织中EG2 阳性细胞密度、Chromotrope 2R阳性细胞的密度及嗜酸性粒细胞活化比率 ,分别与非变应性患者相比较 ,均无显著性差异 (P >0 .0 5 )。结论 :①Chromotrope 2R特染法结合应用EG2 抗体的免疫组化染色法简便易行 ,适合临床应用观察鼻息肉中嗜酸性粒细胞的浸润和活化状况 ;②活化嗜酸性粒细胞在鼻息肉的发病机制中可能起重要作用。

The infiltration and activation stage of eosinophils in nasal polyps

Objective: To assess the infiltration and activation of eosinophils in nasal polyps. Method:Nasal polyps from sixteen patients were investigated. The samples were stained with Chromotrope 2R histochemical and ABC immunohistochemical methods. Result:The majority of eosinophils in nasal polyps were activated, and there were no significant differences in the numbers of total eosinophils (Chromotrope 2R positive cells) and activated eosinophils (EG 2 positive cells) between allergic and nonallergic patients. Conclusion:Eosinophilia is a prominent histologic feature of nasal polyps which indicates that the activated eosinophils may play vital role in the pathogenesis of nasal polyps.