| RNA干扰沉默mdrl基因联合阿霉素对卵巢癌耐药细胞株SKOV3/ADM增殖的抑制作用 |
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| 引用本文： | 王萍玲,胡丽娜,邓凯贤,张君,宋文鑫. RNA干扰沉默mdrl基因联合阿霉素对卵巢癌耐药细胞株SKOV3/ADM增殖的抑制作用[J]. 重庆医科大学学报, 2005, 30(2): 232-235 |
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| 作者姓名： | 王萍玲 胡丽娜 邓凯贤 张君 宋文鑫 |
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| 作者单位： | 重庆医科大学第二临床学院妇产科;重庆医科大学病毒性肝炎研究所,重庆,400010 |
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| 摘 要： | 目的:观察RNA干扰沉默mdrl基因后化疗药物对卵巢癌耐药细胞株SKOV3/ADM增殖的影响.方法:RT-PCR检测靶向于mdrl基因的小发夹状RNA(pshRNA-mdrl)对SKOV3/ADM细胞内mdr1mRNA表达的影响;采用MTT法及软琼脂克隆形成实验测定pshRNA-mdrl联合化疗药物阿霉素对SKOV3/ADM的增殖抑制作用.结果:pshRNA-mdrl能抑制SKOV3/ADM细胞mdr1mRNA的表达,pshRNA-mdr1联合阿霉素明显抑制SKOV3/ADM细胞的增殖.结论:靶向于mdr1的pshRNA联合阿霉素能显著抑制SKOV3/ADM的增殖,增敏化疗.
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| 关 键 词： | 卵巢癌 耐药 RNAi mdr1基因 |
| 文章编号： | 0253-3626(2005)02-0232-04 |
| 修稿时间： | 2004-09-16 |
Effect of silencing mdrl gene by RNA interference with adriamycin on proliferation of SKOV3/ADM |
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| WANG Pingling,et al. Effect of silencing mdrl gene by RNA interference with adriamycin on proliferation of SKOV3/ADM[J]. Journal of Chongqing Medical University, 2005, 30(2): 232-235 |
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| Authors: | WANG Pingling et al |
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| Abstract: | Objective:To investigate the effect of RNA interference targeting mdrl gene with ADM on ovarian cancer resistant strain SKOV 3/ADM.Methods:The expression of mdrlmRNA after transfected pshRNA-mdrl was detected by RT-PCR;MTT test and clone forming test were applied to measure the inhibition combined with adriamycin on proliferation of SKOV 3/ADM strain.Results:These pshRNA-mdrls inhibited the expression of mdrlmRNA and proliferation of SKOV 3/ADM obviously when combined with adriamycin.Conclusion:The short hairpin RNA recombinant plasmid targeting mdrl gene combined with adriamycin inhibits the proliferation of SKOV 3/ADM and increases the sensitivity to chemotherapy. |
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| Keywords: | Ovarian cancer Multidrug resistance (MDR) RNAi mdrl gene |
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