| 电离辐射对人肺腺癌细胞株A549体外表达VEGF的影响 |
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| 引用本文： | 凌春华,苏燎原,陈卫昌,杨吉成,季成.电离辐射对人肺腺癌细胞株A549体外表达VEGF的影响[J].苏州大学学报(自然科学版),2003,23(3):284-285. |
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| 作者姓名： | 凌春华 苏燎原 陈卫昌 杨吉成 季成 |
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| 作者单位： | [1]苏州大学附属第一医院，苏州215006 [2]苏州大学放射医学与公共卫生学院，苏州215007 [3]苏州大学医学院基础医学系，苏州215007 |
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| 摘 要： | 目的探讨电离辐射对人肺腺癌细胞体外表达血管内皮生长因子(VEGF)的影响。方法人肺腺癌细胞株A549，置RPMI 1640培养液培养，分别接受1、2、4、8Gy电离辐射，继续培养细胞，24、48h后收集细胞上清液，双抗体夹心ELISA法测定VEGF。表达水平。结果与阴性处理细胞比较，人肺腺癌细胞株A549体外接受不同剂量辐照后，VEGF表达明显增高，且具有剂量依赖性和时程依赖性。结论电离辐射能诱导肺腺癌细胞VEGF高表达，这可能是临床肿瘤抗辐射的机制之一。
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| 关 键 词： | 肺腺癌 电离辐射 血管内皮生长因子 体外表达 A549 |
| 文章编号： | 1000-5749(2003)03-0284-02 |
| 修稿时间： | 2003年2月12日 |
Effects of Ionizing Radiation on VEGF Expression in A549 Cell Line of Lung Adenocarcinoma |
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| LING Chun-hua ,SU Liao-yuan ,CHEN Wei-chang ,et al.Effects of Ionizing Radiation on VEGF Expression in A549 Cell Line of Lung Adenocarcinoma[J].Suzhou University Journal of Medical Science,2003,23(3):284-285. |
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| Authors: | LING Chun-hua SU Liao-yuan CHEN Wei-chang |
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| Institution: | LING Chun-hua 1,SU Liao-yuan 2,CHEN Wei-chang 1,et al |
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| Abstract: | Objective To investigate the effects of ionizing radiation (IR) on vascular endothelial growth factor (VEGF) expression in human lung adenocarcinoma cells.Methods A549 human lung adenocarcinoma cells were maintained in RPMI 1640 medium and exposed to IR in incremental doses of 1, 2, 4 and 8 Gy. These cells continued to be cultured in the same medium for 24 and 48 hours after exposure, and the media were harvested for measurement of VEGF protein levels by ELISA. Results Exposure of A549 cells to IR induced significant upregulation of VEGF expression. VEGF levels exhibited both an IR dose- and time-dependent increase in comparison with the control unirradiated cells.Conclusion IR-mediated induction of VEGF in lung adenocarcinoma cells may contribute to clinically-related tumor radio-resistance. |
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| Keywords: | ionizing radiation lung adenocarcinoma VEFG |
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