土拨鼠肝炎病毒和乙型肝炎病毒的核心蛋白存在共同线性B淋巴细胞表位

目的寻找并证实土拨鼠肝炎病毒核心抗原（WHcAg）和HBcAg存在共同的B淋巴细胞表位。方法用变性重组WHcAg和HBcAg免疫Balb/c小鼠以制备单克隆抗体；用单克隆抗体对WHcAg和HBcAg进行ELISA、Western blot及免疫组织化学对比分析,同时用该抗体对WHcAg和HBcAg进行表位分析,并在氨基酸水平进行比对。结果经过ELISA、Western blot、免疫组织化学对比分析,制备的单克隆抗体中有2株与HBcAg和WHcAg有交叉反应,而且灵敏度和特异性相似；针对的抗原表位分别位于HBcAg和WHcAg的1～8位氨基酸和125～140位氨基酸。结论WHcAg和HBcAg存在2个共同的线性B淋巴细胞表位,分别位于1～8位和125～140位氨基酸。

Common linear B-cell epitopes in human hepatitis B virus core protein and woodchuck hepatitis virus core protein

Objective To search for and verify some common B cell epitopes in the core proteins of woodchuck hepatitis virus and human hepatitis B virus.Methods Monoclonal antibodies against both core proteins of woodchuck hepatitis virus (WHV) and httman hepatitis B virus (HBV) were prepared by inoculat- ing Balb/c mice with denatured recombination WHV and HBV core proteins.ELISA and immunoblotting assays for WHcAg and HBcAg were carried out by using these antibodies.Immunohistochemistry was car- fled out with liver tissue sections of both WHV-infected woodchucks and chronic HBV-infected patients.The epitopes were mapped with the mouse mAbs (6D1 and IH4) by using a panel of 24 16mer overlapping peptides covering the entire WHcAg.The amino acid sequences of WHcAg and HBcAg were compared. Results Cross-reactions were observed between mAbs (6DI and IH4) and WHcAg and between Mabs and HBcAg/HBcAg in ELISA and immunoblotting assay.Liver tissue sections of both WHV-infected wood- chucks and chronic HBV-infected patients could be stained specifically by mAbs.The epitopes were mapped at aa1-8 (6D1) and aa125-140 (1H4) of the core proteins of both WHV and HBV by using ELISA assay. WHcAg and HBcAg share similar amino acids sequences at aa1-8 and aa125-140 respectively.Contusion The core proteins of woodchuck hepatitis virus and human hepatitis B virus share common linear B cell epitopes which span aal-8 and aa125-140 respectively.