非小细胞肺癌中细胞外信号调节激酶表达的意义

目的 探讨细胞外信号调节激酶ERK1和ERK2蛋白在非小细胞肺癌组织中的表达及其意义。方法 应用固定化蛋白质印迹法检测52例非小细胞肺癌及癌旁正常肺组织中ERK1、ERK2及磷酸化的ERK（P—ERK）蛋白的表达；应用免疫组织化学法分析其在细胞内的定位。结果 肺癌组织中ERK1、ERK2及P—ERK蛋白的表达水平明显高于癌旁正常组织，分别为癌旁组织的1．5、1．8和1．7倍（P〈0．01）；ERK1与ERK2蛋白表达水平呈正线性相关（r=0．64，P〈0．01）。免疫组织化学显示ERK蛋白主要位于细胞浆内。结论 ERK蛋白的过表达可能在非小细胞肺癌的发生、发展过程中起着重要的作用。

Expression of extracelluar signal-regulated kinase in nonsmall cell lung ancer and its clinical significance

Objective To investigate the expression and activation of extracellular signal-regulated kinases (ERK) in lung cancer and its clinical significance.Methods Samples were obtained from 52 patients with nonsmall cell lung cancer.Western blot was used to detect the expression of ERK,ERK2 and P-ERK proteins.Immunohistochemistry was used for localization of ERK protein.Results The levels of ERK1,ERK2 and P-ERK proteins in nonsmall cell lung cancer tissues were increased,which were 1.5,1.8 and 1.7 times compared with those in adjacent normal tissues respectively,and they all had remarkable difference (P<0.01).ERK1 and ERK2 were observed to be a positive linear correlation.Immunohistochemistry revealed that ERK proteins were mainly located in cytoplasm.Conclusion Over-expression of ERK may play an important role in the development of human nonsmall cell lung cancer.