新型OxyPlateTM厌氧系统隔离眼部厌氧菌的评估

目的:厌氧细菌可引起眼部感染,我们测试OxyPlate^TM厌氧系统(OxyPlate^TM Anaerobic System,OXY)隔离可引起眼部疾病的厌氧细菌。方法:OXY不需要直接的厌氧条件（比如厌氧袋,罐）,将其与常规的厌氧袋培养基相比。琼脂培养基上眼部厌氧细菌菌株在好氧和厌氧条件下（厌氧袋）行标准的菌落计数:（1）OXY（好氧）;（2）5%羊血（sheep blood,SB）;（3）巧克力琼脂;（4）Schaedler琼脂。测试的眼部体外培养细菌来自眼内炎,泪囊炎,包括10个丙酸杆菌和3个放线菌种类。在每个培养条件下,每个细菌菌落计数隔离,排名从大到小,并在非参数比较下确定最佳的培养条件。结果:所有的厌氧条件对于厌氧菌株呈阳性反应。厌氧菌在有氧条件下的SB和Schaedler的琼脂中无法增长。痤疮丙酸杆菌在巧克力琼脂中生长稀疏。作为一种厌氧系统,在厌氧袋SB分离比OXY（P=0.0028）和巧克力琼脂（P=0.0028）分离出更多的菌落数。结论:虽然OXY经测试并没比其他的厌氧系统更高效,它似乎是一个合理隔离厌氧细菌的替代方法。其琼脂培养基在一个专门设计的盘并不需要厌氧袋使得OXY优于其他厌氧系统。

Evaluation of the new OxyPlateTM Anaerobic System for the isolation of ocular anaerobic bacteria

AIM: Anaerobic bacteria can cause ocular infections. We tested the OxyPlate^TM Anaerobic System(OXY)to isolate pertinent anaerobic bacteria that can cause ocular disease.

METHODS: OXY, which does not require direct anaerobic conditions(i.e. bags, jars), was compared to conventional isolation of incubating culture media in anaerobic bags. Standard colonies counts were performed on anaerobic ocular bacterial isolates under aerobic and anaerobic conditions(anaerobic bags)using agar media: 1)OXY(aerobic only), 2)5% sheep blood(SB), 3)Chocolate, and 4)Schaedler. The bacteria tested were de-identified ocular isolates cultured from endophthalmitis and dacryocystitis that include 10 Propionibacterium acnes and 3 Actinomyces species. The colony counts for each bacteria isolate, on each culturing condition, were ranked from largest to smallest, and non-parametrically compared to determine the best culturing condition.

RESULTS:All anaerobic conditions were positive for all of the anaerobic isolates. SB and Schaedler's agar under aerobic conditions did not support the growth of anaerobic bacteria. Sparse growth was noted on chocolate agar with Propionibacterium acnes. As an anaerobic system, SB in an anaerobic bag isolated higher colony counts than OXY(P=0.0028)and chocolate agar(P=0.0028).

CONCLUSION: Although OXY did not test to be more efficient than other anaerobic systems, it appears to be a reasonable alternative for isolating anaerobic bacteria from ocular sites. The use of an agar medium in a specially designed plate, without the requirement of an anaerobic bag, rendered OXY as an advantage over other anaerobic systems.