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改良Mallory‘三色法对牙胚矿化过程的观察
引用本文:袁林天,文玲英,蒋维中,杨富生,杨连甲. 改良Mallory‘三色法对牙胚矿化过程的观察[J]. 实用口腔医学杂志, 2001, 17(2): 124-126
作者姓名:袁林天  文玲英  蒋维中  杨富生  杨连甲
作者单位:西安第四军医大学口腔医学院儿童口腔科
摘    要:目的:观察牙釉质和牙本质基质矿化过程的动态变化,为阐明牙轴质和牙本质的形成机制提供依据。方法:21和32周自然流产的胎儿2例。分离含有牙胚的上、下颌骨,固定脱钙,25g/L火棉胶、石腊双重包埋,常规组织学切片,用改良Mallor's三爸和HE染色,镜下观察。结果:在牙胚矿化过程中,不同时期、不同矿化层呈现不同的颜色变化。结论:改良Mallor's三色法较HE染色更为清楚的观察到牙胚发育中组织矿化过程的变化。

关 键 词:改良三色法 牙胚 发育 矿化
文章编号:1001-3733(2001)-02-0124-03
修稿时间:2000-09-26

Mineralization in dental germs observed with modified Mallory's trichrome staining
Yuan Lintian,Wen Lingying,Jiang Weizhong,et al.. Mineralization in dental germs observed with modified Mallory's trichrome staining[J]. Journal of Practical Stomatology, 2001, 17(2): 124-126
Authors:Yuan Lintian  Wen Lingying  Jiang Weizhong  et al.
Affiliation:Yuan Lintian,Wen Lingying,Jiang Weizhong,et al. Department of Pediatric Dentistry Stomatological College,Fourth Military Medical University,Xi'an 710032
Abstract:Objective:To observe the dynamic process of the development of dentin and enamel during their mineralization period. Methods: Dental germs in maxillae and mandibulae were obtained from spontaneously aborted 21 weeks and 32 weeks fetus. The samples were prepared and stained with modified Mallory's trichrome staining and observed under light microscope. Results: Different mineralized layers in different period of development appeared different color variation in the dental germs. Conciusion: Modified Mallory's trichrome staining method may be used to study the mineralization of dental germ.
Keywords:Modified Mallory's trichrome staining methods  Tooth germ  Development  Mineralization
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