BRCA1和RAD51在食管鳞癌组织中的表达及临床意义

目的]探讨乳腺癌易感基因1(breast cancer susceptibility gene 1,BRCA1)和RAD51在食管癌组织中的表达及其意义.方法]采用SP免疫组织化学法检测120例食管鳞癌和癌旁正常组织及60例上皮内瘤变患者BRCA1和RAD51的表达情况并比较,分析BRCA1和RAD51的阳性表达与临床病理特征间的关系,COX回归分析影响食管鳞癌预后的因素.结果]BRCA1在食管鳞癌中阳性表达主要定位于细胞质,BRCA1的阳性表达显著高于上皮内瘤变组织和癌旁正常组织(P<0.05).RAD51在食管鳞癌阳性表达定位于细胞核,其阳性表达明显高于上皮瘤变组织和正常食管黏膜组织(P<0.05).相关性分析显示BRCA1与RAD51在食管鳞癌组织中的表达呈正相关(P<0.05).淋巴结转移和RAD51是影响食管鳞癌患者预后的主要因素.结论]BRCA1和RAD51在食管鳞癌中的阳性表达与患者预后不良有关,有望成为评估食管鳞癌生物学行为新的生物标志物.

Expression of BRAC-1 and RAD51 in Esophageal Squamous Cell Carcinoma and its Clinical Sig nificance

Objective]To determine the expression of BRCA1 and RAD51 in the esophageal squamous cell carcinoma ( ESCC) and its significance. Methods]The expression of BRCA1 and RAD51 in 120 cases of esophageal squamous cell carcinoma and adjacent normal tissues and 60 cases of intraepithelial neoplasia were detected by SP immunohistochemical method.The relationship between the positive expression of BRCA1 and RAD51 and clinicopathological features was analyzed and compared, and the prognostic factors of esophageal squamous cell carcinoma were analyzed by COX regression analysis.Results]The positive expression of BRCA1 in esophageal squamous cell carcinoma was mainly localized in cytoplasm, and the positive expression of BRCA1 was significantly higher than that of intraepithelial neoplasia and adjacent normal tissues ( P<0.05) . The positive expression in esophageal squamous cell carcinoma was located in the nucleus, and the positive ex-pression of RAD51 was significantly higher than that of epithelial and normal esophageal mucosa tissues( P<0.05) . Correla-tion analysis showed that the expression of BRCA1 was positively correlated with the expression of RAD51 in esophageal squamous cell carcinoma ( P<0.05) . Lymph node metastasis and RAD51 are the main factors affecting the prognosis of pa-tients with esophageal squamous cell carcinoma.Conclusion]The positive expression of BRCA1 and RAD51 in esophageal squamous cell carcinoma is associated with poor prognosis, which is expected to be a new biomarker for evaluating the bio-logical behavior of esophageal squamous cell carcinoma.