高效液相色谱法测定火麻仁测中大麻酚的含量

 目的建立火麻仁油中大麻酚的高效液相色谱测定方法。方法高效液相色谱法，用Irregular-H-C₁₈柱(250 mm×4.6 mm,10 μm)，流动相为甲醇-乙腈-水-冰醋酸(64∶15∶21∶0.4)，流速0.8 mL·min^-1，检测波长为220 nm，柱温为室温，外标法定量。结果大麻酚在1.205～7.229μg·mL^-1范围内呈良好的线性关系，r=0.999 8；平均回收率为95.4%，RSD=2.3%(n=9)。结论本方法简便、可靠，重现性好。

Study on determination of cannabinol in the hemp seed oil by HPLC

OBJECTIVE To establish the analytical METHOD for cannabinol in the hemp seed oil by means of high performance liquid chromatography.METHOD Irregular-H-C₁₈(250 mm×4.6 mm,10 μm) column was used.A mixture of methanol acetonitrile water acetic acid (64∶15∶21∶0.4) was used as the mobile phase with a flow rate of 0.8 mL per minute.The detection wavelength was set at 220 nm.The contents were calculated by external standard method.RESULTS The calibration curve for cannabinol was linear over the range of 1.205～7.229 μg·mL^-1(r=0.999 8).The average recovery and RSD were 95.4% and 2.3%(n=9) respectively.CONCLUSION The method appeared to be convenient,reliable and repeatable.