| mdr-1基因转染的骨髓细胞对大剂量MMF治疗的耐受性研究 |
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| 引用本文： | 李钺,栾晓峰,陈勇,龚建平,陈先锋,丁雄,刘作金.mdr-1基因转染的骨髓细胞对大剂量MMF治疗的耐受性研究[J].第三军医大学学报,2010,32(3). |
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| 作者姓名： | 李钺 栾晓峰 陈勇 龚建平 陈先锋 丁雄 刘作金 |
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| 作者单位： | 重庆医科大学附属第二医院肝胆外科,重庆市肝胆外科重点实验室,重庆,400010 |
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| 基金项目： | 中国博士后科学基金(20070411151);;重庆市自然科学基金(CSTC2007BB0266,CSTC2008BB5229);;重庆市涪陵区自然科学基金(2008-48)~~ |
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| 摘 要： | 目的检测转染多药耐药基因-1(multidrug resistance gene-1,mdr-1)的骨髓细胞能否耐受大剂量霉酚酸脂(mycophenolate mofetil,MMF)的杀伤作用,为体内实验提供依据。方法将重组腺病毒Ad-EGFP-mdr-1转染大鼠骨髓细胞。RT-PCR法和Western blot法检测mdr-1 mRNA及蛋白的表达水平;柔红霉素排出试验检测转染mdr-1基因后的骨髓细胞有无功能表达;MTT法检测不同浓度MMF处理后转染mdr-1基因骨髓细胞的存活情况。结果骨髓细胞病毒转染率为30.14%,存活率为89.25%;转染后骨髓细胞mdr-1的mRNA及蛋白质水平都存在功能性表达;导入的mdr-1表达产物P-gp有药物外排泵功能;不同剂量的MMF处理后,转染mdr-1基因的骨髓细胞存活率没有明显差异(P>0.05)。结论转染mdr-1的大鼠骨髓细胞能有效耐受超大剂量MMF治疗。
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| 关 键 词： | 多药耐药基因-1 霉酚酸脂 骨髓细胞 |
Tolerance of mdr-1 transfected bone marrow cells to overdose mycophenolate mofetil |
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| Li Yue,Luan Xiaofeng,Chen Yong,Gong Jianping,Chen Xianfeng,Ding Xiong,Liu Zuojin.Tolerance of mdr-1 transfected bone marrow cells to overdose mycophenolate mofetil[J].Acta Academiae Medicinae Militaris Tertiae,2010,32(3). |
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| Authors: | Li Yue Luan Xiaofeng Chen Yong Gong Jianping Chen Xianfeng Ding Xiong Liu Zuojin |
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| Institution: | Department of Hepatobiliary Surgery;Second Affiliated Hospital;Chongqing Key Laboratory of Hepatobiliary Surgery;Chongqing Medical University;Chongqing;400010;China |
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| Abstract: | Objective To investigate whether bone marrow cells(BMC) transfected with multidrug resistance gene-1(mdr-1) can tolerate to the killing effect of overdose mycophenolate mofetil(MMF).Methods BMC were transfected with recombinant adenovirus,Ad-EGFP-mdr-1.Expression levels of mdr1 mRNA and protein were detected by RT-PCR,Western blotting and daunorubicin(DAM),respectively.The survival of mdr-1 transfected BMC after treatment with MMF at different concentrations was detected by MTT.Results The transfection effi... |
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| Keywords: | multidrug resistance gene-1 mycophenolate mofetil bone marrow cel |
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