康复新液治疗小鼠实验性结肠炎的研究

目的：探讨康复新液对恶唑酮诱导的小鼠实验性结肠炎的影响及其机制。方法：健康昆明小鼠60只分为5组：正常对照组、模型对照组、康复新液A组、康复新液B组、康复新液C组。除正常组外,其余小鼠均以恶唑酮灌肠造模并给予不同形式的药物干预。造模5d后处死,进行疾病活动指数（DAI）、大体和组织学损伤评分,提取肠黏膜固有层单个核细胞（LPMC）,以荧光定量聚酶链反应（PCR）检测表皮生长因子（EGF）、激活蛋白1（AP-1）、核因子κB（NF-κB）、白细胞介素4（IL-4）的表达量。结果：（1）模型组、康复新液A组的DAI、大体和组织学损伤评分均显著高于正常组;康复新液B组则显著低于模型组而更接近于正常组;康复新液C组高于正常组但低于模型组（P〈0.05）;（2）LPMC中模型组和康复新液A组的EGF表达量显著低于正常组;而AP-1、NF-κB、IL-4的表达量均显著高于正常组。康复新液B组EGF的表达量与正常组无显著差异,而AP-1、NF-κB、IL-4的表达量显著低于模型组而接近正常组。康复新液C组EGF的表达量低于正常组,而AP-1、NF-κB、IL-4的表达量低于模型组而高于正常组。结论：康复新液具有促进EGF表达以修复黏膜的作用,且可通过抑制AP-1、NF-κB、IL-4等炎性递质的表达来达到全身及局部的抗炎作用。

Investigation of the Effect of Kangfuxin on Experimental Colitis in Mice

Objective:To investigate the therapeutic effect of Kangfuxin on oxazolone induced colitis in mice.Methods: Sixty mice were randomized into five groups(n=12): normal group,model group,Kangfuxin A group,Kangfuxin B group and Kangfuxin C group.Except the mice in normal group,all mice were induced by oxazolone enema.The mice in three Kangfuxin groups were treated by Kangfuxin in different ways.Disease activity index(DAI) was assessed during the experiment.All mice were sacrificed 5 days after enema and general ...