|
|||||
|
|
| ACE2和ACE在自发性高血压大鼠心肌中的变化 | |
| 引用本文： | 杨震,于欣,贾绍斌,程丽,缪丽燕,李红霞,韩莲花,蒋文平.ACE2和ACE在自发性高血压大鼠心肌中的变化[J].临床心血管病杂志,2011,27(12). |
| 作者姓名： | 杨震 于欣 贾绍斌 程丽 缪丽燕 李红霞 韩莲花 蒋文平 |
| 作者单位： | 1. 宁夏医科大学附属医院心脏中心,银川,750004 2. 宁夏医科大学检验学院 3. 苏州大学第一附属医院心内科 |
| 摘 要： | 目的:观察自发性高血压大鼠(SHR)心肌的血管紧张素转化酶(ACE)和ACE2的表达,以探讨ACE2和ACE在高血压发生发展中的变化。方法:取15只SHR,处死,分离左心室,行RT-PCR、Western blot蛋白质免疫印迹和免疫组织化学检测ACE及ACE2表达;同步取10只WKY大鼠作为正常血压对照组。结果:SHR组心肌ACE的mRNA和蛋白质表达都显著高于WKY组(1.68±0.34)∶(0.33±0.12),P<0.05;(1.21±0.14)∶(0.71±0.11),P<0.05],而ACE2的mRNA和蛋白质表达皆明显低于WKY组(0.50±0.15)∶(1.16±0.24),P<0.05;(0.71±0.24)∶(1.22±0.14),P<0.05)]。免疫组织化学染色显示,SHR组ACE的阳性率明显高于WKY组(87%∶50%,P<0.05),而ACE2的阳性率明显低于WKY组(27%∶70%,P<0.05)。结论:SHR心肌ACE明显升高,ACE2显著降低;SHR高血压发生发展过程中存在着ACE和ACE2表达的失衡。 |
| 关 键 词： | 自发性高血压大鼠 WKY大鼠 血管紧张素转化酶2 血管紧张素转化酶 |
Cardiac expression of angiotensin-converting enzyme 2 and angiotensin-converting enzyme in spontaneously hypertensive rats |
|
| YANG Zhen,YU Xin,JIA Shaobin,CHENG Li,MIAO Liyan,LI Hongxia,HAN Lianhua,JIANG Wenping.Cardiac expression of angiotensin-converting enzyme 2 and angiotensin-converting enzyme in spontaneously hypertensive rats[J].Journal of Clinical Cardiology,2011,27(12). | |
| Authors: | YANG Zhen YU Xin JIA Shaobin CHENG Li MIAO Liyan LI Hongxia HAN Lianhua JIANG Wenping |
| Institution: | YANG Zhen1 YU Xin2 JIA Shaobin2 CHENG Li3 MIAO Liyan3 LI Hongxia3 HAN Lianhua3 JIANG Wenping3(1Heart Center,the Affiliated Hospital of Ningxia Medical University,Yinchuan,750004,China,2School of Laboratory Medicine,Ningxia Medical University,3Department of Cardiology,the First Hospital Affiliated to Suzhou University) |
| Abstract: | Objective:To investigate the cardiac expression of angiotensin-converting enzyme(ACE) and ACE2 in spontaneously hypertensive rats(SHR),whereby to explore the changes of ACE2 and ACE in hypertension.Method:Fifteen SHR were killed and the left ventricles were dissected carefully.RT-PCR,Western blot protein staining and immunohistochemistry stainning were performed to detect the cardiac expression of ACE and ACE2.Ten WKY rats served as the normotensive control group.Result:Compared with the normotensive WKY,th... |
| Keywords: | spontaneously hypertensive rat wistar kyoto rat angiotensin-converting enzyme 2 angiotensin-converting enzyme |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |