| Orexin受体拮抗剂对睡眠剥夺的戊四氮致疒间大鼠癫疒间发作及脑组织病理学变化的影响 |
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| 引用本文： | 倪丽艳,杨位霞,唐吉友,王爱华.Orexin受体拮抗剂对睡眠剥夺的戊四氮致疒间大鼠癫疒间发作及脑组织病理学变化的影响[J].临床神经病学杂志,2011(6). |
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| 作者姓名： | 倪丽艳 杨位霞 唐吉友 王爱华 |
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| 作者单位： | 山东大学附属千佛山医院神经内科;山东省巨野县人民医院;上海中山医院清浦分院神经内科; |
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| 基金项目： | 教育部留学回国人员科研启动基金资助项目(20101108); 山东省自然科学基金(Y2008C161) |
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| 摘 要： | 目的探讨orexin-1受体(OX1R)和orexin-2受体(OX2R)拮抗剂对睡眠剥夺(SD)的戊四氮(PTZ)致疒间大鼠癫疒间发作及脑组织病理学变化的影响。方法雄性Wistar大鼠48只,随机分为正常对照(NC)组、PTZ组、SD+PTZ(SD)组、SD+PTZ+二甲基亚砜(DMSO)组、SD+PTZ+OX1R拮抗剂SB334867(SB)组和SD+PTZ+OX2R拮抗剂TCS OX229(TCS)组。采用改良多平台SD法,SD前及SD 48 h分别给予相应组大鼠侧脑室注射DMSO、SB或TCS。SD 72 h给予各组腹腔注射PTZ 50 mg/kg诱导癫疒间发作;观察各组大鼠癫疒间发作的潜伏期、发作等级评分、发作持续时间及死亡率;应用常规染色法观察海马的病理学变化,免疫荧光法(BrdU标记)观察神经细胞增殖的变化。结果 (1)与PTZ组比较,SD组及DMSO组疒间性发作的潜伏期明显缩短,发作等级评分、持续时间及死亡率明显增加(均P<0.001),海马CA3区神经元损害加重,海马齿状回门区和颗粒细胞下层BrdU阳性细胞数显著增多(P<0.001);SD组与DMSO组间差异无统计学意义。(2)与SD组比较...
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| 关 键 词： | orexin受体 睡眠剥夺 癫疒间 |
Effects of orexin receptor antagonists on seizure and changes of brain tissue pathology in pentylenetetrazol induced epileptic rats following sleep deprivation |
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| NI Li-yan,YANG Wei-xia,TANG Ji-you,et al..Effects of orexin receptor antagonists on seizure and changes of brain tissue pathology in pentylenetetrazol induced epileptic rats following sleep deprivation[J].Journal of Clinical Neurology,2011(6). |
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| Authors: | NI Li-yan YANG Wei-xia TANG Ji-you |
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| Institution: | NI Li-yan,YANG Wei-xia,TANG Ji-you,et al.Department of Neurology,the Qianfoshan Hospital Affiliated to Shandong University,Jinan 250014,China |
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| Abstract: | Objective To investigate the effects of orexin-1 rceptor(OX1R) and orexin-2 rceptor(OX2R) antagonists on seizure and changes of brain tissue pathology in pentylenetetrazol(PTZ) induced epileptic rats following sleep deprivation(SD). Methods Forty-eight male Wistar rats were randomly divided into normal control(NC) group,PTZ group,SD+PTZ(SD) group,SD+PTZ+dimethylsulfoxide(DMSO) group,SD+PTZ+OX1R antagonist SB334867(SB) group and SD+PTZ+OX2R antagonist TCS OX229(TCS) group.SD was made by the modified multipla... |
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| Keywords: | orexin receptor sleep deprivation epilepsy |
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