胞苷脱氨酶基因对小鼠大剂量化疗的保护作用 |
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摘 要: |
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关 键 词: | cytidine deaminase gene therapy Ara-C 胞苷脱氨酶 基因对 小鼠 大剂量 化疗 的保护 作用 mice high dose chemotherapy toxicity resistant gene increase animal survival rate control contrast group transplanted Bone marrow |
Protection of a cytidine deaminase gene gainst toxicity of high dose chemotherapy in mice |
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Authors: | Bo Chen Caigang Liu Yang Lu Ping Lu Feng Jin Fan Yao Ruishan Zhang Jinlong Liu Shicheng Zhao |
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Institution: | Department of Surgical Oncology, The First Affiliated Hospital, China Medical University, Shenyang 110001, China |
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Abstract: | Objective:To explore the feasibility of transfecting cytidine deaminase(CD)gene into mouse bone marrow cells in order to observe the drug resistance of high dose Ara-C and improve the tolerance of myelosuppression following combination chemotherapy.Methods:Human cytidine deaminase gene was transfected into mice bone marrow cells by retroviral vector.Resistant colony-forming unit granulocyte-macrophage(CFU-GM)assay was performed after the transfected mice bone marrow cells treated by the Ara-C.DNA was extracted from mice bone marrow cells.The drug resistant gene in mice bone marrow cells after transfection was detected by PCR.Results:Bone marrow cells of lhe donor mice cultured with lhe retroviral producer cells showed the drug resistant colonies and resistance to Ara-C,so did accept mice transplanted with the CD gene(CFU-GM of donor mice was 52%,X2=124.62,P<0.01:accept mice was 54%,X2=126.26.P<0.01,both compared with the contrast group).The animal survival rate was significantly higher in gene transfected group than that of the control(X2=7.42.P<0.01).CD gene of transfected bone marrow cells was confirmed by PCR.Conclusion:CD gene can be transfected into bone marrow cells of mice efficiently and increase the drug resistance to Ara-C. |
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Keywords: | cytidine deaminase gene therapy Ara-C |
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