联用乳酸杆菌和丁酸梭菌治疗小鼠急性溃疡性结肠炎的实验研究

摘要 目的：观察联用乳酸杆菌和丁酸梭菌对小鼠急性溃疡性结肠炎的疗效并探讨其治疗机制。方法：建立DSS诱导的小鼠急性UC模型，观察给予乳酸杆菌和丁酸梭菌治疗后，小鼠结肠粘膜的病理改变和TNF-α和P-SEL表达的变化。结果：乳酸杆菌和丁酸梭菌可明显减轻小鼠结肠粘膜的损伤；可明显抑制TNF-α和P-SEL的表达，尤以两菌合用组抑制作用最强。结论：乳酸杆菌和丁酸梭菌对DSS诱导的UC有治疗作用，对TNF-α和P-SEL表达的协同抑制作用可能是其发挥协调治疗作用的分子机制。

Experimental study on combining lactobacilli strain with clostridium butyricum strain on acute mice ulcerative colitis

Aim To investigate the therapeutic effect of combination of lactobacilli strain with clostridium butyricum strain on experimental ulcerative colitis. Mothods To observed the effects of lactobacilli strain with clostridium butyricum strain ,the mice ulcerative colitis was induced by Dextran sulfate Sodium(DSS) and tissue biochemical indexes and the expression of tumor necorisis factor(TNF-α) and P-selection (P-SEL) in the colonic mucosa were detected. Results :Lactobacilli strain and clostridium butyricum strain significantly abbreviated the damage of colonic tissue and suppressed the expression of TNF-αand P-SEL .moreover, there were the lightest histological damage and the lowest expression of TNF-αand P-SEL in lactobacilli strain combined with clostridium butyricum strain. Conclusions Both lactobacilli strain and clostridium butyricum strain show therapeutic effect on DSS induced mice ulcerative colitis.The coeffects on ulcerative colitis were observed in combination of lactobacilli strain with clostridium butyricum strain. There are lower expression of TNF- αand P-SEL in the combination group than that in the two strain group alone ,which may be the molecule mechanism of the coeffect on mice UC .