Localization of tachykinin receptors and Fos-like immunoreactivity induced by substance P in guinea-pig brain |
| |
Authors: | Yip J Chahl L A |
| |
Affiliation: | Experimental Pharmacology Unit, School of Biomedical Sciences, University of Newcastle, New South Wales, Australia. |
| |
Abstract: | 1. In the present study, a comparison was made between the distribution of tachykinin NK1 and NK3 receptor immunoreactivity and the distribution of Fos-like immunoreactivity induced by the tachykinin agonist substance P (SP) in the guinea-pig brain. 2. In agreement with results from previous studies in rat brain, NK1 receptor-immunoreactive neurons were found to be widely distributed throughout the brain in the striatum and in diencephalic and mesencephalic structures, while NK3 receptor-immunoreactive neurons were mainly in telencephalic structures. Considerable overlap was observed between NK1 and NK3 receptor distributions. 3. Substance P induced Fos-like immunoreactivity (Fos-LI) in extensive areas of the guinea-pig brain. The induction of Fos-LI was markedly inhibited in many areas by pretreatment with the NK1 receptor antagonist SR 140333. The NK3 receptor antagonist SR 142801 reduced Fos-LI staining in fewer areas, although a reduction was observed in the cortex, striatum and hypothalamus. 4. In general, tachykinin receptors were located at sites corresponding to areas of functional activation by SP, as shown by Fos-LI. These results extend previous studies by adding a functional dimension to tachykinin receptor localization studies. |
| |
Keywords: | c-Fos guinea-pig brain immunohistochemistry NK1 receptor NK3 receptor substance P tachykinin |
本文献已被 PubMed 等数据库收录! |
|