| RNAi沉默肺腺癌A549细胞系PD-L1基因表达对其增殖和凋亡的影响 |
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| 引用本文： | 蒋涛,付立业,李妍. RNAi沉默肺腺癌A549细胞系PD-L1基因表达对其增殖和凋亡的影响[J]. 现代肿瘤医学, 2015, 23(2): 177-180 |
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| 作者姓名： | 蒋涛 付立业 李妍 |
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| 作者单位： | 中国医科大学附属第一医院肿瘤研究所二室,辽宁沈阳,110001 |
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| 摘 要： | 目的:沉默肺癌细胞系A549的程序性死亡配体1(programmed death ligand 1,PD-L1)基因,观察其对A549细胞增殖和凋亡的影响。方法:构建PD-L1 shRNA重组质粒p GPU6/PD-L1,并应用脂质体转染法将其转染入A549细胞。RT-PCR法检测PD-L1基因的表达;Western blotting法检测PD-L1蛋白的表达;MTT法检测p GPU6/PD-L1对A549细胞增殖的影响;AnnexinⅤ-FITC/PI双染法检测p GPU6/PD-L1对A549细胞凋亡的影响。结果:成功将p GPU6/PD-L1转染入A549细胞;RT-PCR结果显示p GPU6/PD-L1使A549细胞PD-L1基因表达水平降低;Western blotting结果显示p GPU6/PD-L1转染A549细胞使PDL1蛋白表达减少;MTT结果显示p GPU6/PD-L1能够抑制A549细胞增殖;AnnexinⅤ-FITC/PI双染法检测结果显示p GPU6/PD-L1能够诱导A549细胞凋亡。结论:p GPU6/PD-L1能够下调肺癌细胞A549 PD-L1的表达,抑制细胞增殖,并诱导细胞凋亡。
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| 关 键 词： | 肺腺癌 PD-L1 凋亡 RNAi |
Knockdown the expression of PD-L1 affects cell proliferation and apoptosis in human lung adenocarcinoma cell line A549 |
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| Jiang Tao,Fu Liye,Li Yan. Knockdown the expression of PD-L1 affects cell proliferation and apoptosis in human lung adenocarcinoma cell line A549[J]. Journal of Modern Oncology, 2015, 23(2): 177-180 |
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| Authors: | Jiang Tao Fu Liye Li Yan |
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| Affiliation: | Jiang Tao;Fu Liye;Li Yan;Second Laboratory of Tumor Research Institute,First Affiliated Hospital,China Medical University; |
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| Abstract: | |
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| Keywords: | lung adenocarcinoma PD-L1 apoptosis RNAi |
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