芦丁抑制家兔血小板激活因子诱导血小板活化作用的实验研究 |
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引用本文: | 陈文梅,金鸣,吴伟,李金荣,臧宝霞,朴永哲.芦丁抑制家兔血小板激活因子诱导血小板活化作用的实验研究[J].中国中西医结合杂志,2002,22(4):283-285. |
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作者姓名: | 陈文梅 金鸣 吴伟 李金荣 臧宝霞 朴永哲 |
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作者单位: | 北京市心肺血管疾病研究所药理研究室,北京,100029 |
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基金项目: | 国家自然科学基金课题(No.39670884),北京市跨世纪科技人才工程及北京市高科技实验室课题资助项目 |
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摘 要: | 目的:观察芦丁对家兔血小板激活因子(PAF)诱导血小板聚集、5-羟色胺(5-HT)释放、血小板内游离钙离子浓度的影响及其机理。方法:以比浊法测定家兔洗涤血小板(WRR)聚集率,邻苯二甲醛(OPT)荧光分光光度法测定5-HT含量,以Fura-2荧光分光光度法测定血小板游离钙离子浓度的变化。结果:芦丁体外呈浓度依赖性抑制PAF(9.55×10^-9mol/L)诱发的WRP聚集、5-HT释放作用,IC50分别为0.73、1.13mmol/L;同时68.3、274、545μmol/L芦丁剂量依赖地抑制PAF(4.78×10^-9mol/L)引起的血小板内游离钙浓度增高。结论:芦丁可抑制PAF诱导的血小板聚集、5-HT释放及血小板内游离钙浓度增加。
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关 键 词: | 血小板聚集 5-羟色胺 游离钙 芦丁 血小板激活因子 血小板活化 实验研究 |
修稿时间: | 2001年3月20日 |
Experimental Study on Inhibitory Effect of Rutin against Platelet Activation Induced by Platelet Activating Factor in Rabbits |
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Authors: | CHEN Wen mei JIN Ming WU Wei |
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Abstract: | OBJECTIVE: To explore the inhibitory effect and mechanism of rutin against platelet activating factor (PAF) induced platelet aggregation, 5-HT release and intra-platelet free calcium concentration. METHODS: The rate of washed rabbit platelet (WRP) aggregation was measured by turbidimetry and O-phthaldialdehyde (OPT) fluoro-spectrophotometry (FSPM) was used to determine 5-HT content. The intraplatelet free calcium concentration was measured with Fura-2/AM FSPM assay. RESULTS: Rutin in vitro was concentration-dependently inhibiting PAF (9.55 x 10(-9) mol/L) induced WRP aggregation, the IC50 of 5-HT release was 0.73, 1.13 mmol/L respectively and the intraplatelet free calcium concentration elevation evoked by PAF (4.78 x 10(-10) mol/L) were inhibited by 68.3, 136, 274, 545 mumol/L of rutin dose-dependently. CONCLUSION: Rutin could inhibit PAF induced platelet aggregation, 5-HT release and the increase of intraplatelet free calcium. |
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Keywords: | rutin platelet aggregation platelet activating factor 5 HT free calcium |
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