Low-molecular-weight proteinase inhibitor of human epidermis inhibiting papain and trypsin activity |
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| Authors: | I. Takiuchi H. Takagi H. Goi M. Kawamura D. Higuchi |
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| Affiliation: | (1) Department of Dermatology, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Midoriku, 227 Yokohama, Japan |
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| Abstract: | Summary A low-molecular-weight proteinase inhibitor was isolated from human epidermal extracts by means of ultrafiltration, gel filtration and ion-exchange chromatography. On polyacrylamide gel electrophoresis (PAGE), the isolated fraction exhibited a single band of activity at pH 9.4, and inhibitory activity against papain and trypsin was detected in this band. Using sodium-dodecyl-sulphate PAGE, the molecular mass of the inhibitor was estimated to be 2,200 daltons. |
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| Keywords: | Low-molecular-weight proteinase inhibitor Human epidermis Papain Trypsin |
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