改构体和野生型aFGF对肠缺血-再灌注损伤保护作用的比较研究

目的：观察外源性给予改构体酸性成纤维细胞生长因子（acid fibroblast growth factor，aFGF）后，大鼠小肠功能指标的变化规律，探讨改构体aFGF对肠缺血-再灌注损伤的影响。方法：以大鼠肠系膜上动脉（SMA）夹闭造成肠缺血-再灌注损伤模型，并将动物随机分为假手术组、生理盐水对照组、改构体aFGF治疗组和野生型aFGF治疗组。除假手术组外，其余各组动物均于缺血45min后于2、6、12、24h活杀，取血度小腑组织标本，检测血浆中D-乳酸含量度组织中增殖细胞棱抗原（PCNA）的表达特性。结果：血浆D-乳酸变化及HE染色显示，再灌注后6h屏障功能损伤最严重，而改构体aFGF治疗组损伤在伤后24h较生理盐水对照组有所减轻。PCNA的表达趋势与D-乳酸类似。结论：改构体aFGF对肠缺血-再灌注损伤具有一定保护与促修复作用。

EFFECTS OF RECONSTRUCTIVE HUMAN AND WILD TYPE ACIDIC FIBROBLAST GROWTH FACTOR ON THE GUT ISCHEMIA-REPERFUSION INJURY IN RATS

Objective: To identify the regulation of change of the plasma D-lactate in rat intestine after the administration of acidic fibroblast growth factor(aFGF),to explore the effects of fibroblast growth factor on the gut ischemia-reperfusion(I/R) injury in rats.Methods: Rats I/R injury was produced by clamping the superior mesenteric artery(SMA),and the animals were divided randomly into sham-operation group,normal saline control group,reconstructive human aFGF treatment group,wild type aFGF treatment group.Blood samples and tissue specimens were collected at 2,6,12,and 24 hours after reperfusion.The levels of plasma D-lactate and the expression of proliferating cell nuclear antigen(PCNA) were detected.Results: The high activity of plasma D-lactate and pathological changes indicated that the intestinal barrier was damaged seriously 6 hours after reperfusion.However,the improvement in histological structures was observed 24 hours after reperfusion in reconstructive human aFGF treatment group and wild type aFGF treatment group.Conclusion: Reconstructive human aFGF may play an important role in intestinal wound healing after injury.