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| 从桂黔交界地区中华硬蜱中分离到莱姆病螺旋体 | |
| 引用本文： | 李永学,王昭孝,曾霞,王树声,余春,周敬祝. 从桂黔交界地区中华硬蜱中分离到莱姆病螺旋体[J]. 中国媒介生物学及控制杂志, 2010, 21(3): 238-240 |
| 作者姓名： | 李永学 王昭孝 曾霞 王树声 余春 周敬祝 |
| 作者单位： | 1. 浙江省医学科学院病毒病研究所,杭州,310013 2. 贵州省疾病预防控制中心 3. 广西医科大学 4. 广西壮族自治区疾病预防控制中心 |
| 基金项目： | 志谢：蜱种的鉴定工作由军事医学科学院微生物流行病研究所许荣满研究员和孙毅博士完成,特此志谢 |
| 摘 要： | 目的从桂黔交界地区捕获的蜱中分离莱姆病螺旋体,以探查广西壮族自治区（广西区）和贵州省莱姆病的传播媒介。方法用BSK-Ⅱ培养基从蜱中分离莱姆病螺旋体。用PCR扩增分离菌株的5S～23SrRNA基因间隔区,将PCR扩增产物克隆后测序,通过将测序结果与基因库中莱姆病螺旋体菌株的5S～23SrRNA基因间隔区进行同源性比较和分析,从而鉴定分离株的基因型。结果从捕获的中华硬蜱中分离出1株莱姆病螺旋体（命名为QLT1）。分离株经鉴定为Borrelia valaisiana基因型莱姆病螺旋体。结论中华硬蜱很有可能是广西区和贵州省莱姆病的传播媒介。 |
| 关 键 词： | 莱姆病 伯氏疏螺旋体 中华硬蜱 基因型 |
Borrelia burgdorferi isolated from Ixodes sinensis in the border areas between Guangxi and Guizhou in China |
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| LI Yong-xue,WANG Zhao-xiao,ZENG Xia,WANG Shu-sheng,YU Chun,ZHOU Jing-zhu. Borrelia burgdorferi isolated from Ixodes sinensis in the border areas between Guangxi and Guizhou in China[J]. Chinese Journal of Vector Biology and Control, 2010, 21(3): 238-240 | |
| Authors: | LI Yong-xue WANG Zhao-xiao ZENG Xia WANG Shu-sheng YU Chun ZHOU Jing-zhu |
| Affiliation: | 1 Institue of Viral Diseases,Zhejiang Academy of Medical Sciences,Hangzhou 310013,Zhejiang Province,China;2 Guizhou Center for Disease Control and Prevention;3 Guangxi Medical University;4 Guangxi Center for Disease Control and Prevention ) |
| Abstract: | Objective Borrelia burgdorferi were isolated from captured ticks in the border areas between Guangxi and Guizhou,in order to identify the vector of Lyme disease in Guangxi Zhuang Autonomous Region and Guizhou province.Methods The BSK-Ⅱ culture media were used to isolate B.burgdorferi from ticks.The 5S-23S rRNA gene spacer region of the isolates was amplified by the PCR,and the product was cloned and sequenced.The sequencing results were compared with the 5S-23S rRNA gene spacer region of the B.burgdorferi strains from the gene bank and analyzed for the homology to identify their genotypes.Results One B.burgdorferi was isolated from the captured Ixodes sinensis（named QLT1）.The isolate was identified as the genotype B.valaisiana.Conclusion I.sinensis is the likely vector of Lyme disease in Guangxi Zhuang Autonomous Region and Guizhou province. |
| Keywords: | Lyme disease Borrelia burgdorferi Ixodes sinensis Genotype |
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