人支气管上皮细胞转化过程中MTS1/p16基因丢失的研究

目的观察人支气管上皮细胞系Ｍ转化过程中ＭＴＳ１／ｐ１６基因的缺失及Ｐ１６蛋白的表达情况。方法采用双色荧光原位杂交,免疫组织化学和Ｗｅｓｔｅｒｎｂｌｏｔ技术。结果在早期ＭＰ１８细胞中没有发现ＭＴＳ１／ｐ１６基因的缺失,在较晚期的ＭＰ３６和ＭＰ９７细胞中ＭＴＳ１／ｐ１６基因均发生了缺失;免疫组织化学和Ｗｅｓｔｅｒｎｂｌｏｔ的结果都表明,晚期Ｍ细胞ＭＰ１２０中Ｐ１６蛋白的表达水平比早期代数细胞ＭＰ２０明显下降。结论ＭＴＳ１／ｐ１６基因的功能失活在人支气管上皮细胞系Ｍ的转化过程中可能起重要作用。

The studies on MTS1/p16 gene deletion during transformation of human bronchial epithelial cells

Objective To detect the deletion of MTS1/p16 and the expression of P16 protein during the transformation of human bronchial epithelial cell line M. Methods Dual color fluo rescence in situ hybridization (FISH), immunohistochemical staining and western blot analysis were carried out. Results No deletion of MTS1/p16 gene was found in MP18 cells of the cell line M. But partial deletions occurred in the MP36 and MP120 cells of the cell line M. Immunohistochemical analysis and western blot test showed lower expression of P16 protein in the MP120 cells than in MP20 cells. Conclusions MTS1/p16 gene deletion contributes to the malignant transformation of the human bronchial epithelial cell line M. Also FISH is a useful technique in detecting gene deletion.