白血病p16基因纯合子缺失的研究

为探讨白血病ｐ１６基因缺失的发生率及其与疾病预后的关系。对４８例初发的白血病患者用多重聚合酶链反应（ＰＣＲ）方法扩增ｐ１６基因外显子１及外显子２，进行ｐ１６基因缺失研究。４８例患者中有ｐ１６基因缺失者４例，分别为：Ｍ２２例，非何杰金淋巴瘤（ＮＨＬ）并发急性Ｂ淋巴细胞白血病（Ｂ ＡＬＬ）、慢性髓性白血病（ＣＭＬ）急淋变各１例。有ｐ１６缺失的４例患者均病情进展迅速，治疗效果差，患者在短期内死亡。提示：ｐ１６基因缺失在部分白血病的发生、发展中起重要作用；有ｐ１６基因缺失者预后较差

Homozygous Deletion of p16 Gene in Leukemia

To investigate the incidence of p16 gene homozygous deletion in leukemia, and the relations between p16 gene homozygous deletion and the prognosis of leukemia, 48 patients were studied by multiplex polymerase chain reaction(PCR) to detect p16 gene homozygous deletion. P16 gene homozygous deletion were detected in 4 of the 48 patients. They were two patients with M 2, one patient with NHL accompany with ALL, and one patient with CML in blast crisis, respectively. P16 gene homozygous deletion correlates with adverse prognosis features. The patients with p16 homozygous deletion had poor response to chemotherapy, and died shortly after p16 gene homozygous deletion was detected. The homozygous deletion of p16 gene palys an improtent role in the pathogenesis and the development of some leukemia. The patients with p16 gene homozygous deletion have a poor prognosis.