| 改进血清铜蓝蛋白凝胶电泳——氧化酶活性盐酸联苯胺显示法 |
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| 引用本文： | 苏燕 邵国. 改进血清铜蓝蛋白凝胶电泳——氧化酶活性盐酸联苯胺显示法[J]. 包头医学院学报, 1999, 15(2): 29-31 |
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| 作者姓名： | 苏燕 邵国 |
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| 作者单位： | 包头医学院血红蛋白研究室!内蒙古包头014010 (苏燕，邵国，秦文斌)，包头医学院血红蛋白研究室!内蒙古包头014010(睢天林) |
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| 摘 要： | 目的：改进血清铜蓝蛋白（ＣＰ）的检测方法。方法：不连续淀粉－琼脂糖凝胶电泳分离血清ＣＰ，利用其氧化酶活性使 苯胺氧化，形成的有色产物沉积物凝胶板上ＣＰＲ 相应位置，可直接观察ＣＰ量的变化。结果：通过比较各种联苯胺的显色时间及显色效果。从而筛选出一种更为有效的血清ＣＰ测定方法。结论：氧化酶活性盐酸联苯胺显示法是最有效的血清ＣＰ检测方法。
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| 关 键 词： | 铜蓝蛋白 联苯胺 凝胶电泳 肝豆状核变性 诊断 |
Improving Gel Electrophorewsis for Detecting Serum Ceruloplasmin with Benzidine Dihgdrochl-oride |
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| Su Yan,Shao Guo,Qin Wenbin,et al. Improving Gel Electrophorewsis for Detecting Serum Ceruloplasmin with Benzidine Dihgdrochl-oride[J]. Journal of Baotou Medical College, 1999, 15(2): 29-31 |
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| Authors: | Su Yan Shao Guo Qin Wenbin et al |
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| Abstract: | Objective: To improve electrophoresis methocl for detecting cerulop lasmin (cp) in serum methods:starch-agaroee gel electrophoresis was used to separate CP from the serum With the oxidase activity of CP ,benzidine was oxidised to form a colored component which deposited where CP exists had existed and changes in CP content could be observed directly Results:Several benzidine's coloration time and effect were observed snd a more effective method was chosen to detecting CP in serum Conclusion :The method of Benzidine Dihydrochloride is the best way to detect cp in serum |
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| Keywords: | ceruloplasmin benzidine gel electrophoresis |
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