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In vitro susceptibility testing of amphotericin B for Cryptococcus neoformans variety grubii AFLP1/VNI and Cryptococcus gattii AFLP6/VGII by CLSI and flow cytometry |
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| Authors: | Bernardina Penarrieta Morales Luciana Trilles Álvaro Luiz Bertho Ivan Neves Junior Raquel de Vasconcellos Carvalhaes de Oliveira Bodo Wanke Márcia dos Santos Lazéra |
| Affiliation: | 1. Laboratory Medical Mycology, National Institute of Infectious Diseases Evandro Chagas Oswaldo Cruz Foundation (INI‐FIOCRUZ), Rio de Janeiro, Brazil;2. Laboratory of Immunoparasitology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation (IOC‐FIOCRUZ), Rio de Janeiro, Brazil;3. Department of Epidemiology and Statistics, National Institute of Infectious Diseases Evandro Chagas Oswaldo Cruz Foundation (INI‐FIOCRUZ), Rio de Janeiro, Brazil |
| Abstract: | Cryptococcus neoformans var. grubii AFLP1/VNI is the main causative agent of cryptococcosis associated with AIDS in the world. Cryptococcus gattii AFLP6/VGII causes mainly endemic primary infection in immunocompetent hosts. To determine the minimum inhibitory concentrations (MICs) of C. neoformans var. grubii AFLP1/VNI and C. gattii AFLP6/VGII against amphotericin B (AMB) in a short period of time, flow cytometry (FCM) with FUN‐1 fluorochrome was used to compare with broth microdilution method (CLSI M27‐A3). The minimum incubation period was evaluated by minimum fungicidal concentration procedure. Seventeen clinical isolates of C. neoformans var. grubii AFLP1/VNI and 18 of C. gattii AFLP6/VGII were analysed. The time for the determination of MICs by FCM was 2 h against 72 h by CLSI M27‐A3 and the comparison of MIC showed a positive significant correlation (P = 0.048). It is important to highlight the role of the FCM as an alternative method to determine the MICs for AMB in within a day, with positive cost‐benefit. |
| Keywords: | Cryptococcus neoformans Cryptococcus gattii amphotericin B CLSI M27‐A3 flow cytometry |